植物保护2026，Vol.52Issue(2)：332-341,10.DOI:10.16688/j.zwbh.2025222
黄瓜绿斑驳花叶病毒随病残体在土壤中越冬越夏后的致病性
Pathogenicity of cucumber green mottle mosaic virus in soil with diseased debris after overwintering and oversummering
摘要
Abstract
Cucumber green mottle mosaic virus(CGMMV)is an important agricultural quarantine pathogen in China.Infection by CGMMV poses potential soil health risks due to virus persistence in plant debris.To determine the survival duration of CGMMV in soil,clarify whether the virus can overwinter and oversummer with infected plant debris under natural conditions in different regions,and assess its potential as a primary inoculum source for subsequent crop,soil extracts containing diseased plant debris were prepared.The pathogenicity of residual virus under different debris-to-soil mass ratios(1.3％,3％,and 6％)was evaluated using mechanical inoculation assays and soil transmission experiments.Under non-overwintering conditions,mechanical inoculation of watermelon with soil extracts at a debris-to-soil ratio of 1.3％resulted in a maximum disease incidence of 86.7％,whereas a ratio of 6％consistently caused 100％disease incidence.Correspondingly,soil transmission rates at debris-to-soil ratios of 1.3％,3％,and 6％were 0,1.5％,and 10.7％,respectively,indicating that virus accumulation in soil must reach a threshold level to enable transmission.In Nanning,Urumqi,and Zhengzhou,soil samples containing 6％diseased plant debris exhibited disease incidence rates of 26.67％,35.33％,and 26.67％,respectively,in watermelon following mechanical inoculation with soil extracts,with soil transmission rates of 2.00％,1.33％,and 1.67％.After overwintering and oversummering,disease incidence decreased to 4.00％,2.00％,and 2.67％,respectively,and soil transmission capacity was almost completely lost.These results indicate that CGMMV can overwinter and oversummer with infected plant debris in cucurbit-producing regions across different latitudes in China and can serve as a primary inoculum source for subsequent cucurbit crops.However,the pathogenicity of residual virus in soil gradually decreases over time.关键词
黄瓜绿斑驳花叶病毒/病残体/土壤传播/越冬越夏Key words
cucumber green mottle mosaic virus/plant debris/soil transmission/overwintering and oversummering分类
农业科技引用本文复制引用
梁智玲,彭斌,刘莉铭,王婉婷,陈红运,古勤生,康保珊..黄瓜绿斑驳花叶病毒随病残体在土壤中越冬越夏后的致病性[J].植物保护,2026,52(2):332-341,10.基金项目
中国农业科学院农业科技创新工程(CAAS-ASTIP-2022-ZFRI-09) （CAAS-ASTIP-2022-ZFRI-09）
河南省重点研发与推广专项(252102110208) （252102110208）
海关总署科研项目(2024HK174) （2024HK174）
