中西医结合肝病杂志2026，Vol.36Issue(3)：298-303,6.DOI:10.3969/j.issn.1005-0264.2026.003.007
槲芪方及其拆方治疗原发性肝癌的机制研究
Investigation into the mechanisms of HuQi Formula and its deconstructed groups for Hepatocellular Carcinoma treatment
摘要
Abstract
Objective:To investigate the antitumor activity of HuQi Formula and its prescription-disassembled groups against hepatocellular carcinoma(HCC)and preliminarily explore its underlying mechanism.Methods:A subcutaneous xenograft model of HCC was established in mice,and intragastric administration was adopted to evaluate the anti-HCC effect of HuQi Formula.Based on the medicinal efficacies,the formula was divided into three prescription-disassembled groups:the Kidney-Tonifying and Qi-Invigorating Group(Mistletoe,Raw Astragalus),the Blood-Activating and Stasis-Removing Group(Danshenmiltiorrhiza,Curcuma zedoaria),and the Heat-Clearing and Detoxifying Group(Hedentaria diffusa,Phyllanthus urinaria).The in vivo antitumor activity of HuQi Formula and each of its disassembled groups was investigated.Meanwhile,peripheral blood and HCC tissues of mice were collected,and immune-and inflammation-related indicators in peripheral blood were detected to preliminarily elucidate the mechanism of action.Results:HuQi Formula and all its disassembled groups significantly inhibited the growth of subcutaneous HCC xenografts in mice.The Blood-Activating and Stasis-Removing Group and the Heat-Clearing and Detoxifying Group exerted antitumor effects by downregulating the expression of inflammation-related cytokines such as IL-6 and IL-17A.The Kidney-Tonifying and Qi-Invigorating Group enhanced the body's immune function by increasing the percentages of CD4+T cells,CD8+T cells and CD8+IFN-γ+T cells in mouse peripheral blood and reducing the levels of Th2-type cytokines.On this basis,HuQi Formula further decreased the expression of VEGF,an indicator associated with HCC metastasis and recurrence,in mouse peripheral blood.Conclusion:HuQi Formula and its prescription-disassembled groups exhibit favorable in vivo antitumor activity against HCC.HuQi Formula exerts its antitumor effects through multiple pathways including enhancing immune function and alleviating inflammatory response,and the compatibility of the compound formula can improve the therapeutic effect.关键词
肝细胞癌/槲芪方/H22细胞/免疫功能/炎症因子Key words
Hepatocellular carcinoma/HuQi Formula/H22 cells/Immune function/Inflammatory factors分类
医药卫生引用本文复制引用
陈欢,袁慧鑫,李伟华,李秀惠..槲芪方及其拆方治疗原发性肝癌的机制研究[J].中西医结合肝病杂志,2026,36(3):298-303,6.基金项目
北京市自然科学基金面上项目(No.7212171) （No.7212171）
