安徽农学通报2026，Vol.32Issue(9)：94-98,5.DOI:10.16377/j.cnki.issn1007-7731.2026.09.023
地参多糖(LLP-1)调控细胞周期的网络药理学和分子对接研究
Network pharmacology and molecular docking studies on the regulation of cell cycle by Lycopus lucidus polysaccharide(LLP-1)
摘要
Abstract
Lycopus lucidus polysaccharide(LLP-1)is the main active component of the medicinal herb Lycopus lucidus,demonstrates considerable application potential in improving soil conditions and enhancing the disease resistance of livestock and poultry.Based on the structural characteristics of the LLP-1,this study used the TargetNet,SwissTargetPrediction and SEA databases for target prediction;selected the genes related to the prognosis of liver cancer from the TCGA database,and conducted intersection analysis with the predicted targets;constructed a protein-protein interaction network(PPI)using the STRING database,identified related biological processes and signaling pathways through GO and KEGG enrichment analysis;and verified the correlation between the core target's prognosis survival curve and immune infiltration;finally,molecular docking was used to validate the affinity of LLP-1 to the target protein.The results showed that the prediction identified 85 potential targets that shared 19 common targets with 6 767 liver cancer-related genes.The PPI network revealed close interactions among the targets,and LASSO regression identified 6 core genes(LGALS3,CCNA2,APH1A,CDK1,CDK2,HSP90AA1).The prognostic model had moderate performance(AUC>0.6).GO enrichment was found in pathways such as positive regulation of DNA metabolism,G2/M and G1/M transitions of the cell cycle;KEGG enrichment was in the cell cycle and cancer pathways.Molecular docking revealed that the binding energy of LLP-1 to the core target was less than-24.00 kJ/mol,among which the binding energy with CCNA2 was the strongest(-42.26 kJ/mol).In summary,the ginseng polysaccharide LLP-1 may exert anti-liver cancer activity by regulating cell cycle-related targets and signaling pathways,and may also regulate the tumor immune microenvironment.This article provides a reference for the development of functional foods containing Lycopus lucidus polysaccharides.关键词
地参多糖/网络药理学/肝癌/预后模型/分子对接Key words
Lycopus lucidus polysaccharide/network pharmacology/hepatocellular carcinoma/prognostic model/molecular docking分类
化学化工引用本文复制引用
廖宇星,梁会晴,杨雯杰,张翠香,黄红梅,杨棋榕,龚嘉棋,陈贵元,李雪英..地参多糖(LLP-1)调控细胞周期的网络药理学和分子对接研究[J].安徽农学通报,2026,32(9):94-98,5.基金项目
国家自然科学基金项目(31860252) （31860252）
云南省自然科学基金地方高校联合面上项目(202301BA070001-043,202401BA070001-089) （202301BA070001-043,202401BA070001-089）
云南省教育厅科学研究基金项目(2025J0767) （2025J0767）
大理州科技重点研发计划专项(20242903B030014) （20242903B030014）
云南省昆虫生物医药研发重点实验室开放项目(AG2022006). （AG2022006）
