安徽农业大学学报2026，Vol.53Issue(1)：48-54,7.DOI:10.13610/j.cnki.1672-352x.20251229.001
海水酸化对口虾蛄生化组成和消化酶活性的鉴定分析
Analysis of the effects of seawater acidification on the biochemical composition and digestive enzyme activities of Oratosquilla oratoria
摘要
Abstract
[Objective]This study aimed to investigate the effects of CO2-induced seawater acidification on the biochemical composition and digestive enzyme activities of mantis shrimp(Oratosquilla oratoria).[Method]A 49-day aquaculture experiment was conducted.Three acidified treatment groups(pH 7.90,pH 7.70,pH 7.50)were established by introducing CO2 gas into seawater,using natural seawater(pH 8.10)serving as the control.[Result](1)Under acidified conditions,the muscle moisture content of O.oratoria increased with decreasing pH levels.From day 21 onward,the moisture content in the pH 7.70 and pH 7.50 groups exhibited significantly high-er than that in the control group(P＜0.05).Conversely,the contents of ash,crude protein,and crude lipid contents decreased with pH reduction.All acidified groups displayed significantly lower ash content than the control from day 7 onward(P＜0.05).The crude protein levels in respective acidified groups became significantly lower than those in the control starting on days 28(pH 7.90 group),14(pH 7.70 group),and 7(pH 7.50 group),respectively(P＜0.05).All acidified groups showed significantly reduced crude lipid content compared to the control from day 7 onward(P＜0.05).(2)Pepsin and trypsin activities showed an initial increase followed by a decrease,while lipase activity exhibited a gradual increasing trend.Although acidified groups exhibited marginally higher pepsin and trypsin activities than the control at day 7,these enzymes showed declining trends subsequently,with signifi-cant inhibition observed in pepsin by day 21 and trypsin by day 35,respectively(P＜0.05).Lipase activities in the acidified groups became significantly elevated compared to the control starting on day 28(pH 7.90 group),day 7(pH 7.70 group),and day 7(pH 7.50 group),respectively(P＜0.05).[Conclusion]When seawater acidification reached pH levels of 7.50-7.30,it induced a stress response in the mantis shrimp(Oratosquilla oratoria).After 7-14 days of exposure,energy reserves such as crude fat and crude protein were mobilized to cope with the acidic conditions.Although the activities of digestive enzymes including pepsin,trypsin,and lipase were temporarily enhanced,this response failed to alleviate the stress caused by seawater acidification.关键词
海水酸化/口虾蛄/生化组成/消化酶/环境胁迫Key words
seawater acidification/Oratosquilla oratoria/biochemical composition/digestive enzyme activ-ity/environmental stress分类
农业科技引用本文复制引用
张泽林,陈雷,孙珮琦,温锦,李想,王佳宁,薛佳欣,常一凡,秦玉雪,尹增强..海水酸化对口虾蛄生化组成和消化酶活性的鉴定分析[J].安徽农业大学学报,2026,53(1):48-54,7.基金项目
辽宁省教育厅基本科学研究面上项目(JYTMS20230491) （JYTMS20230491）
辽宁省科技计划项目(2024JH2/102600082) （2024JH2/102600082）
农业农村部渔业渔政管理局政府购卖服务合同项目(09250010) （09250010）
