安徽农业科学2026，Vol.54Issue(8)：149-156,186,9.DOI:10.3969/j.issn.0517-6611.2026.08.026
基于基因组和代谢组学的冬虫夏草菌虫草素生物合成代谢途径研究
Study on the Biosynthetic Pathway of Cordycepin in Ophiocordyceps sinensis Based on Genomics and Metabolomics
摘要
Abstract
[Objective]To investigate the biosynthetic pathway of cordycepin in Ophiocordyceps sinensis,and provide a theoretical basis for in-depth research and biotechnological application[Method]High-throughput sequencing technology was used to sequence and assemble the whole genome of O.sinensis.Potential key enzyme genes involved in cordycepin biosynthesis were predicted and identified through comparative ge-nomic analysis.The metabolic profile of O.sinensis fruiting bodies was analyzed by liquid chromatography-mass spectrometry(LC-MS),identif-ying differential metabolites associated with cordycepin synthesis.Furthermore,the possible biosynthetic pathway of cordycepin was inferred by integrating key gene functions,dynamic changes of differential metabolites,and KEGG pathway enrichment analysis.[Result]Chromosome-level genome assembly and preliminary analysis revealed that the O.sinensis genome size is 111.4 Mbp with a GC content of 45.43%and an N50 of 17.66 Mbp.A total of 9 519 protein-coding genes were identified,with an average length of 1 481 bp.Enzymes such as adenylate ki-nase,adenosine kinase,and ribonucleic acid reductase and 5'-nucleotidase were predicted to be involved in cordycepin biosynthesis.[Con-clusion]Through genomic and metabolomic integration analysis,the biosynthetic pathway network of cordycepin in O.sinensis was initially constructed at the systems biology level,and multiple key candidate genes and metabolic nodes were identified,laying a theoretical foundation for further verification of functional genes and the production of cordycepin using synthetic biology strategies.关键词
冬虫夏草菌/虫草素/生物合成/代谢途径Key words
Ophiocordyceps sinensis/Cordycepin/Biosynthesis/Metabolic pathway分类
生物科学引用本文复制引用
贺元川,王嘉,卫秋阳,陈康,胡印,陈仕江,刘翔..基于基因组和代谢组学的冬虫夏草菌虫草素生物合成代谢途径研究[J].安徽农业科学,2026,54(8):149-156,186,9.基金项目
重庆市级科研院所绩效激励引导专项(cstc2023jxjl-jbky-X0002) （cstc2023jxjl-jbky-X0002）
重庆市技术创新与应用发展专项重点项目(CSTB2024TIAD-KPX0049) （CSTB2024TIAD-KPX0049）
重庆市中药研究院院管项目(zzkjjh2022007) （zzkjjh2022007）
重庆英才计划创新创业领军人才项目(CQYC20220301627). （CQYC20220301627）
