阿尔茨海默病及相关病2026，Vol.9Issue(2)：83-89,7.DOI:10.3969/j.issn.2096-5516.2026.02.002
探讨小续命汤抑制阿尔茨海默病体外细胞模型铁自噬的作用研究
Study on the inhibitory effect of Xiao-Xu-Ming decoction on ferritinophagy in Alzheimer's disease in vitro cell model
摘要
Abstract
Objective:To observe the effects of Xiao-Xu-Ming Decoction(XXMD)on the expression of nuclear receptor coactivator 4(NCOA4)and ferritin heavy chain(FTH1)in glutamate(Glu)-induced neurotoxic injury in mouse hippocampal neuronal cells(HT22),and to explore the mechanism of traditional Chinese medicine in treating Alzheimer's disease(AD).Methods:HT22 cells in the logarithmic growth phase were divided into a control group,a Glu model group,and low-and high-dose XXMD groups.An in vitro AD cell model was established by inducing HT22 cell damage with 20 mmol/L Glu.The cells in the XXMD groups were treated with different concentrations(500 and 1 000 μg/mL)of XXMD.The effects of XXMD on the proliferation and survival rate were detected using the methyl thiazolyl tetrazolium(MTT)assay.Autophagy flux was assessed using mRFP-GFP-LC3 adenovirus transfection.The associations between NCOA4/LC3 and NCOA4/FTH1 expression were examined by immunofluorescence double staining.The protein expression levels of LC3B Ⅱ/Ⅰ,NCOA4,and FTH1 were measured using Western blot analysis.Results:XXMD alleviated Glu-induced damage to HT22 cells in a dose-dependent(P<0.01).Compared with the control group,the Glu group showed increased autophagy flux,elevated protein expression of NCOA4 and LC3B Ⅱ/Ⅰ(P<0.01),and decreased protein expression of FTH1(P<0.01).Compared with the Glu group,the low-and high-dose XXMD groups exhibited reduced autophagy flux,decreased protein expression of NCOA4 and LC3B Ⅱ/Ⅰ(P<0.01),and upregulated FTH1 protein expression(P<0.01).Conclusions:XXMD significantly improves the survival rate of Glu-induced HT22 cells and inhibits abnormal autophagy flux.The mechanism may involve the downregulation of NCOA4 protein expression and upregulation of FTH1 protein expression.关键词
小续命汤/阿尔茨海默病/核受体共激活因子4/铁蛋白重链Key words
Xiao-Xu-Ming decoction/Alzheimer's disease/NCOA4/FTH1分类
医药卫生引用本文复制引用
李文涛,赵悦,陈锦团,苏晓燕,毛敬洁..探讨小续命汤抑制阿尔茨海默病体外细胞模型铁自噬的作用研究[J].阿尔茨海默病及相关病,2026,9(2):83-89,7.基金项目
福建省自然科学基金资助项目(2022J01841) （2022J01841）
