北方园艺Issue(7)：29-37,9.DOI:10.11937/bfyy.20253273
枣疯病对酸枣中类黄酮生物合成途径的差异基因表达分析
Analysis of Differential Gene Expression in the Flavonoid Biosynthesis Pathway of Jujube in Jujube Witches' Broom Disease
摘要
Abstract
Taking jujube witches' disease-resistant healthy leaves(KB)and disease-susceptible leaves(ZFB)as experimental materials,the flavonoid content of KB and ZFB was determined by NaNO2-Al(NO3)3-NaOH colorimetric method.Transcriptome sequencing technology and qRT-PCR technology were used to screen for metabolic pathways and differentially expressed genes related to resistance to jujube disease,and quantitative verification was performed.The effects of jujube witches'-broom phytoplasma on the flavonoid biosynthesis pathway of sour jujube were studied,in order to provide reference for the breeding of sour jujube varieties.The results showed that a total of 4 682 differentially expressed genes(DEGs)were collected between KB and ZFB,of which 1 540 genes were upregulated and 3 142 genes were downregulated.In GO enrichment analysis,a total of 4 066 differentially expressed genes were annotated,including flavonoid biosynthesis and flavonoid metabolism processes.In the KEGG enrichment pathway,it was also significantly enriched in pathways such as flavonoid biosynthesis.Based on the annotations of 4 682 DEGs in the transcriptome database,25 key genes related to flavonoid biosynthesis were identified,including HCT and CCoAOMT genes,which were significantly upregulated in the KB vs ZFB group and could promote the accumulation of ferulic acid coenzyme A.There were 226 transcription factor family genes,mainly concentrated in ERF,MYB,bHLH families known to regulate flavonoid metabolism.关键词
酸枣/枣疯病/转录因子/类黄酮生物合成Key words
Ziziphus jujuba var.spinosa/jujube witches' broom disease/transcription factors/flavonoid biosynthesis分类
农业科技引用本文复制引用
王超慧,尤兴天,庞建英,刘云鸽,马东来,宋永兴..枣疯病对酸枣中类黄酮生物合成途径的差异基因表达分析[J].北方园艺,2026,(7):29-37,9.基金项目
石家庄市市级科技计划资助项目(241200013A) （241200013A）
河北省高等学校科学研究基金资助项目(QN2026206) （QN2026206）
河北中医药管理局科研计划资助项目(2024094) （2024094）
河北省三期现代农业产业技术体系创新团队资助项目(HBCT2024110201). （HBCT2024110201）
