滨州医学院学报2026，Vol.49Issue(2)：121-126,6.DOI:10.19739/j.cnki.issn1001-9510.2026.02.002
敲低MALAT1对人鼻咽癌HONE-1细胞增殖、迁移和侵袭的影响
Long chain non-coding R NA MALAT1 gene knockdown inhibits growth and migration and invasion of HO NE-1 of human nasopharyngeal carcinoma cells
摘要
Abstract
Objective To investigate the effect of MALAT1 knockdown on the proliferation and migration and invasion in HONE-1 of nasopharyngeal carcinoma cells.Methods The nasopharyngeal carcinoma HONE-1 cells were divided into two groups,which were transfected with control plasmid(shcon group)and shMALAT1 plasmid(shMALAT1 group)respectively.The transfection rates of the two groups were detected by fluorescence microscope.And MALAT1 gene knockdown level was de-tected by real-time fluorescence quantitative PCR(qRT-RCR).The proliferation abilities were detected by CCK8 method;the migration and invasion ability of the two groups were detected by transwell assay;and the expression levels of vimentin and E-cadherin mRNA were detected by qRT-PCR;and the expression levels of vimentin and E-cadherin protein were detected by West-ern blot.Results Compared with the control group,the expressions level of MALAT1 was significantly decreased after transfection shMALAT1 plasmid.Compared with the control group,the cell proliferation ability(t=5.520,P＜0.000 1)and migration a-bility(t=9.285,P＜0.000 1;t=9.577,P＜0.000 1)and invasion ability(t=8.481,P＜0.000 1)in shMALAT1 group was significantly decreased,the expressions level of vimentin mRNA and protein in the shMALAT1 group were significantly de-creased.the expression levels of E-cadherin mRNA and protein in the shMALAT1 group were significantly increased.Conclusion Knocking down MALAT1 gene can inhibit proliferation,migration,invasion and EMT of HONE-1 cells in human nasopharyn-geal cancer.关键词
鼻咽癌/肺腺癌转移相关转录本 1/细胞增殖/细胞迁移/细胞侵袭/上皮-间充质转化Key words
metastasis-associated lung adenocarcinoma transcript 1/nasopharyngeal carcinoma/cell proliferation/cell migra-tion/cell invasion/epithelial-mesenchymal transition分类
医药卫生引用本文复制引用
王丹丹,周宁,安迪,蒋佳彤,郑琪,李泽明,武艳,刘冬芹,代娟娟..敲低MALAT1对人鼻咽癌HONE-1细胞增殖、迁移和侵袭的影响[J].滨州医学院学报,2026,49(2):121-126,6.基金项目
国家自然科学基金(81903102) （81903102）
山东省自然科学基金(ZR2023MH126) （ZR2023MH126）
山东省医药卫生科技发展计划(202202080906,202501041056) （202202080906,202501041056）
滨州医学院科研计划与科研启动基金(BY2019KJ04) （BY2019KJ04）
滨州医学院"临床+X"项目(BY2021LCX23) （BY2021LCX23）
