草业学报2026，Vol.35Issue(4)：54-66,13.DOI:10.11686/cyxb2025170
有机肥和生物炭添加对亚热带人工草地土壤微生物碳、磷限制的缓解作用
Mitigation of soil microbial carbon and phosphorus limitations through organic fertilizer and biochar inputs in subtropical cultivated grassland
摘要
Abstract
The aim of this research was to investigate the effects of fertilization on soil enzyme activities and soil enzyme stoichiometry in subtropical cultivated grassland.We conducted a field experiment in which organic fertilizer and biochar were applied to subtropical grassland.Then,changes in soil physicochemical properties were monitored and the impacts of these inputs on soil enzyme activities and enzyme stoichiometric indices were determined.Additionally,microbial nutrient limitations were assessed through determination of the stoichiometric characteristics of soil extracellular enzyme activities.The results show that the application of organic fertilizer and biochar to subtropical cultivated grassland significantly increased the soil organic carbon and total nitrogen contents,as well as soil pH(P＜0.05).The application of organic fertilizer significantly increased β-1,4-glucosidase activity in the 0-10 cm,10-20 cm,and 20-30 cm soil layers by 36.18％,37.21％,and 59.30％,respectively(P＜0.05).It also increased β-1,4-N-acetyl aminidinidase activity in the 0-10 cm,10-20 cm,and 20-30 cm soil layers by 21.16％,17.25％,and 30.24％,respectively(P＜0.05).Application of biochar significantly increased leuine ameptiase and β-1,4-N-acetyl aminidinidase activity in all three soil layers(P＜0.05).The average ratio of C∶N∶P for enzyme acquisition in subtropical cultivated grassland soil was 1∶1.31∶1.72,deviating from 1∶1∶1,indicating that microorganisms in this area are phosphorus-limited.After fertilization,the vector length and vector angle decreased,suggesting that fertilization can alleviate carbon and phosphorus limitations in the soil of subtropical grassland.These findings provide a theoretical basis for the improvement and management of subtropical cultivated grassland.Our results show that the appropriate application of organic fertilizers or biochar can contribute to the enhancement and restoration of subtropical artificial grassland,thereby alleviating carbon and phosphorus limitation in soil.关键词
土壤酶活性/土壤酶化学计量/养分限制/有机肥/生物炭Key words
soil enzyme activity/soil enzyme stoichiometry/nutrient limitation/organic fertilizer/biochar引用本文复制引用
刘畅,陈积山,朱瑞芬,孙万斌,姚博,董世魁..有机肥和生物炭添加对亚热带人工草地土壤微生物碳、磷限制的缓解作用[J].草业学报,2026,35(4):54-66,13.基金项目
国家重点研发计划(2022YFD1300805)和重庆市级财政资金-畜牧科技研究与推广项目(酸性土壤主要饲草PGPR菌筛选及促生性能比较)资助. （2022YFD1300805）
