草业学报2026，Vol.35Issue(4)：135-146,12.DOI:10.11686/cyxb2025167
基质栽培对兰州百合茎生小鳞茎发育的激素调控机制研究
Hormonal regulation of stem-derived bulblet development in Lilium davidii var.unicolor cultivated in a peat-perlite substrate
摘要
Abstract
The aim of this work was to explore the effects of the cultivation substrate on bulb proliferation of Lanzhou lily(Lilium davidii var.unicolor)and to clarify its hormonal regulation mechanisms.Lanzhou lily plants were grown in two substrates:pure sandy loam soil(CK)and a mixture of peat and perlite at a 1∶1 ratio(T1).During the experiment,growth indices of Lanzhou lily were measured,and the endogenous hormone contents in developing bulblets were detected by ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The results show that the T1 treatment significantly promoted the growth of Lanzhou lily.At the flowering stage(June 15),compared with the plants in CK,those in the T1 treatment showed 13.5％greater plant height(41.47 cm vs.36.53 cm)and 22.6％greater stem diameter(7.97 mm vs.6.50 mm).The number of bulblets was significantly higher in the T1 treatment than in CK.The contents of methyl jasmonate(4.76 ng·g-1),N6-isopentenyladenine(0.63 ng·g-1),and gibberellin(GA)A12 aldehyde(3.04 ng·g-1)were significantly higher in plants in the T1 treatment than in those in the CK(1.90,0.30,and 0.90 ng·g-1,respectively)(P＜0.05).The contents of tryptamine(1.69 ng·g-1)and salicylic acid-2-O-β-glucoside(229.71 ng·g-1)were significantly lower in plants in the T1 treatment than in those in the CK(3.54 and 530.19 ng·g-1,respectively)(P＜0.05).Metabolic pathway analysis indicated that the differentially accumulated hormones were mainly enriched in 20 pathways including plant hormone signal transduction and diterpenoid biosynthesis.Among the metabolites of these pathways,GA A12 aldehyde and N6-isopentenyladenine were identified as potential key regulatory nodes in bulb development.The results of this study confirmed that the peat∶perlite=1∶1 substrate significantly promoted the proliferation of Lanzhou lily caudex bulblets(P＜0.05)by regulating the synthesis and transport of cytokinin,gibberellin,and jasmonic acid.These findings provide a theoretical basis for the large-scale production of Lanzhou lily bulblets.关键词
兰州百合/栽培基质/茎生小鳞茎/内源激素Key words
Lilium davidii var.unicolor/cultivation substrates/cauline bulblets/endogenous hormones引用本文复制引用
马祥臣,勉鹏飞,张萍,李不染,靳磊..基质栽培对兰州百合茎生小鳞茎发育的激素调控机制研究[J].草业学报,2026,35(4):135-146,12.基金项目
国家自然科学基金(32260727)和宁夏回族自治区重点研发计划项目(2021BBF02025)资助. （32260727）
