大豆科学2026，Vol.45Issue(2)：13-24,12.DOI:10.11861/j.issn.1000-9841.2026.02.0013
大豆S40家族生物信息学分析及盐碱干旱胁迫下表达模式分析
Bioinformatics Analysis of Soybean S40 Family and Expression Analysis Under Abiotic Stress
摘要
Abstract
The S40 gene family belongs to the DUF584 family,and its encoded proteins contain the DUF584 domain.To elucidate the function of the soybean S40 gene in responses to abiotic stresses such as salt-alkali and drought,this study conducted a genome-wide identification of genes containing the DUF584 domain in the soybean genome,and systematically carried out analyses including the construction of a family evolutionary tree,domain composition,gene structure,cis-acting elements in the promoter,synteny analysis,and tissue-specific expression.Finally,the expression patterns of these genes under salt-alkali,drought,and osmotic stress were detected.The results showed that a total of thirty S40 family members were identified in the soybean genome,distributed on 13 chromosomes.Physical and chemical property analysis indicated that the amino acid lengths of the encoded proteins of the soybean S40 gene family ranged from 49 to 266 aa,and the molecular weights were between 5.43 and 28.96 kDa.Phylogenetic analysis classified the S40 genes of soybean,Arabidopsis,and rice into five subfamilies,among which the G1 and G5 subfamilies had the largest number of members.Domain analysis revealed that all S40 members contained only the conserved DUF584 domain,but the positions of the domain in the sequences were different,suggesting possible functional diversification among the family members.Gene structure analysis showed that the number and arrangement of exons and introns in different members were significantly different,further supporting their functional diversity.Promoter cis-acting element analysis found that S40 family members contained light response,growth and development,hormone regulation,and stress-related elements,suggesting that this family is involved in the response of soybean to abiotic stresses.Synteny analysis indicated that members within the same subfamily had closer genetic relationships and higher conservation.Expression pattern analysis under adverse conditions showed that the expression level of the G5 subfamily gene GmS40-3 changed most significantly under salt-alkali,drought,and osmotic stress.This study provides a theoretical basis for the functional analysis of the soybean S40 gene family and also offers reference for further clarifying the mechanism of soybean adaptation to adverse conditions and improving its stress tolerance.关键词
大豆/S40/生物信息学分析/非生物胁迫/表达分析Key words
soybean/S40/bioinformatics analysis/abiotic stress/expression analysis引用本文复制引用
张业奇,刘洋,张滨烁,韩敬佩,武小霞,赵莹..大豆S40家族生物信息学分析及盐碱干旱胁迫下表达模式分析[J].大豆科学,2026,45(2):13-24,12.基金项目
国家重点研发计划(2023YFD2300101-02) （2023YFD2300101-02）
黑龙江省自然科学基金(YQ2022C010,TD2022C003). （YQ2022C010,TD2022C003）
