南方医科大学学报2026，Vol.46Issue(4)：861-870,10.DOI:10.12122/j.issn.1673-4254.2026.04.14
金天格胶囊通过抑制血管内皮细胞衰老并促进骨形成以缓解大鼠糖皮质激素性骨质疏松
Jintiange Capsule alleviates glucocorticoid-induced osteoporosis in rats by inhibiting vascular endothelial cell senescence and promoting bone formation
摘要
Abstract
Objective To investigate the role of cellular senescence in glucocorticoid-induced osteoporosis and explore the therapeutic mechanism of Jintiange(JTG)Capsule.Methods Cultured human umbilical vein endothelial cells(HUVECs)and mouse pre-osteoblasts(MC3T3)were treated with methylprednisolone(MPS)or MPS combined with JTG Capsule dissolved in saline.HUVEC senescence pathways and repair capacity were analyzed using Western blotting,SA-β-Gal staining,DCFH-DA assay,scratch wound healing,and RT-PCR.The osteogenic potential of MC3T3 cells was assessed using immunofluorescence staining,ALP staining,Alizarin Red S staining,and RT-PCR.Osteoclast differentiation was evaluated by TRAP staining.Thirty 3-week-old female SD rats were randomized into control,MPS,and MPS+JTG groups,and the rats in the latter two groups received daily intraperitoneal MPS injections and treated with gavage of saline or JTG Capsule suspension for 3 months.Femurs and venous blood were collected from the rats for micro-CT analysis of femoral bone volume fraction and detection of serum bone metabolism markers.Results Network pharmacology revealed that the active components of tiger bone had numerous intersection targets with glucocorticoid-induced osteoporosis with a strong binding affinity to cellular senescence-related targets such as P53.In MPS-treated HUVEC-derived osteoclasts,treatment with JTG Capsule significantly inhibited the expressions of P53 and P21,attenuated oxidative stress,and enhanced cell migration and angiogenic capacity.Similarly,JTG Capsule significantly enhanced osteogenic and mineralization capacities of MC3T3 cells and suppressed osteoclast differentiation.In the rat models,intraperitoneal MPS injection for 14 days resulted in significant bone loss in the femur,and JTG Capsule treatment obviously alleviated bone loss and ameliorated the disorder in serum bone metabolic markers.Conclusion JTG Capsule alleviates MPS-induced HUVEC senescence,enhances osteogenic capacity and suppresses osteoclastogenesis in MC3T3 cells,and promotes intraosseous angiogenesis to improve glucocorticoid-induced osteogenesis inrats.关键词
金天格胶囊/糖皮质激素性骨质疏松/细胞衰老/血管再生/成骨分化Key words
Jintiange Capsule/glucocorticoid-induced osteoporosis/cell senescence/angiogenesis/osteogenic differentiation引用本文复制引用
邓子豪,王转,蒙森雄,杨玉生,杨运平,范刘奕,王磊..金天格胶囊通过抑制血管内皮细胞衰老并促进骨形成以缓解大鼠糖皮质激素性骨质疏松[J].南方医科大学学报,2026,46(4):861-870,10.基金项目
国家自然科学基金(82002352) （82002352）
金天格中青年科研培养基金(项目103) Supported by National Natural Science Foundation of China(82002352). （项目103）
