安全与环境工程2026，Vol.33Issue(2)：346-356,11.DOI:10.13578/j.cnki.issn.1671-1556.20250178
农田连续免耕对甲烷氧化菌及甲烷减排的影响
Impact of continuous no-tillage farming on methane-oxidizing bacteria and methane emission reduction
摘要
Abstract
No-tillage agriculture effectively reduces methane emissions from soil,but the impact of continuous no-tillage on methane emissions remains unclear.This study investigated farmland soils in Wenling City,Zhejiang Province,China,by analyzing surface soil samples from fields with four different no-tillage years.We examined the differences in methane emission flux,methane oxidation rate,and the relative abundance,community composition,and diversity of methane-oxidizing bacteria under varying continuous no-tillage years.By integrating soil physicochemical factors,we identified key environmental factors influencing methane reduction in continuously no-tillage soils.The results show that continuous no-tillage within a certain period effectively reduces methane emission flux while increasing the methane oxidation rate and the relative abundance of methane-oxidizing bacteria in soil.In conventionally tilled soils,Type Ⅰ methane-oxidizing bacteria(Methylococcaceae)dominate,whereas Type Ⅱ methane-oxidizing bacteria(Methylocystaceae)prevail in no-tillage soils.Organic matter and ammonium nitrogen content show significant positive correlations with methane-oxidizing bacteria abundance,particularly with the relative abundance of Type Ⅱ methane-oxidizing bacteria(Methylocystaceae),but a negative correlation with the relative abundance of Type Ⅰ methane-oxidizing bacteria(Methylococcaceae).These findings indicate that organic matter and ammonium nitrogen are key environmental factors influencing methane-oxidizing bacteria community structure and methane emissions in continuous no-tillage soils.These findings provide a theoretical basis for formulating farming practices that reduce greenhouse gas emissions in agricultural ecosystems.关键词
连续免耕/甲烷排放通量/甲烷氧化菌/pmoA功能基因/高通量测序Key words
continuous no-tillage/methane emission flux/methane-oxidizing bacteria/pmoA functional gene/high-throughput sequencing分类
资源环境引用本文复制引用
吴泽宇,殷汉琴,龚冬琴,谷安庆,张嵩乐,王艳红,李平..农田连续免耕对甲烷氧化菌及甲烷减排的影响[J].安全与环境工程,2026,33(2):346-356,11.基金项目
国家自然科学基金项目(42177066) （42177066）
自然资源部平原区农用地生态评价与修复工程技术创新中心课题项目(2022316602) （2022316602）
