福建农业科技2026，Vol.57Issue(2)：70-77,8.DOI:10.13651/j.cnki.fjnykj.2026.02.009
猴头菇粗多糖复合益生菌发酵提取工艺优化及抗氧化活性分析
Process Optimization and Antioxidant Activity Analysis of Crude Polysaccharide Extracted from Hericium erinaceus by Compound Probiotics Fermentation
摘要
Abstract
In order to optimize the key processing parameters for extracting crude polysaccharide from Hericium erinaceus by the fermentation of compound probiotics,this paper aimed to improve the extraction rate of crude polysaccharides and clarify their antioxidant activity.By using the single factor and orthogonal experiment methods,the effects of strain ratio,addition amount,temperature,time and initial pH value on the extraction rate of crude polysaccharide from Hericium erinaceus during the fermentation of compound probiotics were discussed,and the antioxidant activity of crude polysaccharide from Hericium erinaceus after the fermentation of compound probiotics was analyzed.The results showed that:compared with the fermentation without probiotics,the extraction rate of crude polysaccharide after the fermentation with compound probiotics(Lactobacillus plantarum∶Bacillus subtilis∶Streptococcus thermophilus was at a ratio of 1∶2∶1)was the highest(16.11%).Through the optimization of single factor and orthogonal experiments,the extraction rate of crude polysaccharide from Hericium erinaceus could reach 18.51%under the conditions of compound probiotic addition amount of 25%,fermentation temperature of 34℃,fermentation time of 12 h,and initial pH of 6.5.When the concentration of crude polysaccharide from Hericium erinaceus was 3.0 mg·mL-1,the scavenging rates for DPPH,hydroxyl and ABTS free radicals were 71.26%,86.90%and 84.59%,respectively.关键词
猴头菇/粗多糖/复合益生菌发酵/抗氧化活性Key words
Hericium erinaceus/Crude polysaccharides/Compound probiotic fermentation/Antioxidant activity分类
轻工纺织引用本文复制引用
黄雅萍,李诗博,陈寿辉,谢勇,肖志勇..猴头菇粗多糖复合益生菌发酵提取工艺优化及抗氧化活性分析[J].福建农业科技,2026,57(2):70-77,8.基金项目
福建省中青年教师教育科研项目(科技类)(JAT220662). （科技类）
