福建农业科技2026，Vol.57Issue(2)：95-99,5.DOI:10.13651/j.cnki.fjnykj.2026.02.012
非衍生化液质联用方法测定啤酒中癸酸和辛酸的含量
Determination of Decylic Acid and Octylic Acid Contents in Beer by Non-derivatization Liquid Chromatography-mass Spectrometry
摘要
Abstract
A non-derivatized liquid chromatography-mass spectrometry(LC-MS)method was established for the determination of decylic acid and octylic acid in beer.The beer samples were extracted with n-hexane at a ratio of 1∶1(V/V),and the supernatant fluid was dried and re-dissolved in methanol.The determination was carried out by using the selected ion monitoring(SIM)mode of LC-MS.The linearity,detection limit,quantification limit,precision and recovery of decylic acid and octylic acid were investigated.The results showed that decylic acid and octylic acid could be well separated under the above chromatographic conditions.There was a good linear relationship between the peak area and the concentration.The linear range of decylic acid was 0.1-15 μg·mL-1 with r=0.997 5,and that of octylic acid was 0.4-50 μg·mL-1 with r=0.9994.The detection limit and quantification limit of decylic acid and octylic acid were both satisfactory for the sample determination.The method had good precision.The average recoveries of decylic acid and octylic acid in beer were 94.5%and 98.9%(n=9),respectively.In conclusion,this method was validated and could be used for the determination of decylic acid and octylic acid in beer.The contents of decylic acid and octylic acid in the six beer samples from 4 manufacturers were determined by this method,with the content range of decylic acid being 0.280 3-1.741 μg·mL-1 and that of octylic acid being 1.722-4.860 μg·mL-1.关键词
癸酸/辛酸/液相色谱-单四极杆质谱联用方法/非衍生化Key words
Decylic acid/Ooctylic acid/LC-MS(SIM)method/Non-derivatized分类
轻工纺织引用本文复制引用
吴酮,王夏,陈楠楠,徐冰,周璟明..非衍生化液质联用方法测定啤酒中癸酸和辛酸的含量[J].福建农业科技,2026,57(2):95-99,5.基金项目
福建省科技计划省属公益类项目(2022R1005008). （2022R1005008）
