福建畜牧兽医2026，Vol.48Issue(2)：41-47,7.
不同核酸提取方法对肉松和肉丸中非洲猪瘟病毒微滴式数字PCR检测结果的影响
The impact of different nucleic acid extraction methods on droplet digital PCR detection results of African swine fever virus in pork floss and meatballs
郑人浩 1邱希斌 2周轲3
作者信息
- 1. 福建农林大学林学院 福州 350002
- 2. 福建省产品质量检验研究院/国家加工食品质量检验检测中心(福州)福州 350015||福建师范大学生命科学学院 福州 350117
- 3. 福州千烨生物科技有限公司 福州 350108
- 折叠
摘要
Abstract
In order to develop a droplet digital PCR method for detection of African swine fever virus in deep processed meat products such as meat floss and meatballs,the aim was to study the effect of different extraction methods on the extraction efficiency of African swine fever virus DNA.By comparing the effects of different nucleic acid extraction methods on the droplet digital PCR detection re-sults of the virus.The methods evaluated included the phenol-chloroform DNA extraction method,the rapid extraction method using the TaKaRa MiniBEST Viral RNA/DNA Extraction Kit,the rapid extraction method using the QIAGEN DNeasy○R mericon Food Kit,and the rapid extraction method using the TIANGEN Namp Genomic DNA Kit.TaKaRa MiniBEST Viral RNA/DNA Extraction Kit and QIAGEN DNeasy○R mericon Food Kit have a relatively good effect.Based on the complexity of the extraction program and the influ-ence of the heating and cracking process,it was ultimately confirmed that the TaKaRa MiniBEST Viral RNA/DNA Extraction Kit rapid extraction method is the most suitable solution.关键词
肉松/肉丸/非洲猪瘟病毒/微滴式数字PCR/DNA提取Key words
Pork floss/Meatballs/African swine fever virus/Droplet digital PCR/DNA extraction引用本文复制引用
郑人浩,邱希斌,周轲..不同核酸提取方法对肉松和肉丸中非洲猪瘟病毒微滴式数字PCR检测结果的影响[J].福建畜牧兽医,2026,48(2):41-47,7.
