分析化学2026，Vol.54Issue(3)：329-338,10.DOI:10.19756/j.issn.0253-3820.251310
基于CRISPR-Cas12a与等温扩增技术融合的致病菌检测方法的研究进展
Research Progress on Pathogen Detection Based on Integration of CRISPR-Cas12a and Isothermal Amplification Technology
摘要
Abstract
Rapid and accurate detection of pathogenic bacteria is of great significance for public health security,disease control,and food safety assurance.In recent years,the clustered regularly interspaced short palindromic repeats-associated proteins 12a(CRISPR-Cas12a)system has emerged as a key tool for pathogenic bacteria detection due to its specific recognition via cis-cleavage and signal amplification capability through trans-cleavage.However,its application is restricted by challenges such as difficulty in detecting low-abundance targets and dependence on protospacer adjacent motif(PAM)sequences.Isothermal amplification technology can rapidly amplify target nucleic acids at a constant temperature,forming a complement to CRISPR-Cas12a.Combining CRISPR-Cas12a with isothermal amplification technology can effectively improve the sensitivity,specificity,and operational convenience of detection,making it suitable for POCT requirements.In this review,the molecular recognition and cleavage mechanisms of Cas12a were firstly outlined,and the characteristics and compatibility of various isothermal amplification methods were summarized.And the recent advances in their combined applications for pathogenic bacteria detection were reviewd,while the current limitations were discussed.Finally,the perspectives on future directions to guide further development in this field was provided.关键词
成簇规律间隔短回文重复序列及其相关蛋白12a/等温扩增/致病菌检测/评述Key words
Clustered regularly interspaced short palindromic repeats-associated proteins 12a/Isothermal amplification/Pathogen detection/Revirew引用本文复制引用
蒙颖乐,刘晓侠,郭隆华,彭育勃,徐建国..基于CRISPR-Cas12a与等温扩增技术融合的致病菌检测方法的研究进展[J].分析化学,2026,54(3):329-338,10.基金项目
浙江省自然科学基金重点项目(No.LZ25C200005)和安徽省自然科学基金项目(No.2308085MB57)资助. Supported by the Zhejiang Provincial Natural Science Foundation(No.LZ25C200005)and the Anhui Provincial Natural Science Foundation(No.2308085MB57). （No.LZ25C200005）
