分析化学2026，Vol.54Issue(3)：372-380,中插11-中插13,12.DOI:10.19756/j.issn.0253-3820.251235
基于苯并噻唑的比率型荧光探针用于高灵敏检测色胺和食品新鲜度
A Colorimetric Fluorescent Probe Based on Benzothiazole for Highly Sensitive Detection of Tryptamine and Food Freshness
摘要
Abstract
Tryptamine,as an important indole-based biogenic amine,is widely present in food and biological organisms.Its excessive accumulation is one of the key factors leading to food poisoning and health risks.Therefore,developing efficient and specific tryptamine detection methods is of great significance for food safety monitoring.In this study,based on the intramolecular charge transfer(ICT)mechanism,a novel ratio-type fluorescent probe NR-1 was designed and synthesized with a benzoyl ester as a specific recognition group.When reacting with tryptamine,the probe showed a ratio-type fluorescence response,with fluorescence weakening at 378 nm and significantly enhancing at 472 nm.The probe showed good selectivity and detection sensitivity for tryptamine,with a detection limit(3σ)of 0.26 μmol/L.Further,the probe NR-1 was loaded onto a cotton swab carrier and combined with smartphone fluorescence imaging to achieve real-time and visual monitoring of tryptamine release in shrimp,chicken wings,mangoes and other foods during storage,and a quantitative relationship between fluorescence signals and storage time was thus established.This method provided a reliable new strategy for in-situ and portable detection of food freshness.关键词
苯并噻唑类荧光探针/比率荧光检测/可视化/色胺/食品新鲜度Key words
Benzothiazole-based fluorescent probes/Ratiometric fluorescence detection/Visualization/Tryptamine/Food freshness引用本文复制引用
李莎,雷皓珺,李雅琪,闫苗,任海仙,温维..基于苯并噻唑的比率型荧光探针用于高灵敏检测色胺和食品新鲜度[J].分析化学,2026,54(3):372-380,中插11-中插13,12.基金项目
山西省自然科学基金项目(No.202303021222234)、忻州市科技计划重点研发项目(No.20250306)和忻州师范学院校级项目(No.XJ2025117)资助. Supported by the Natural Science Foundation of Shanxi Province(No.202303021222234),the Xinzhou City Science and Technology Project Fund(No.20250306)and the Xinzhou Normal University Fund(No.XJ2025117). （No.202303021222234）
