广东农业科学2026，Vol.53Issue(2)：67-79,13.DOI:10.16768/j.issn.1004-874X.2026.02.005
LaPCBER1基因在'Tresor'百合鳞茎发育过程中的功能解析
Functional Analysis of LaPCBER1 Gene During the Development of Bulbs of Lilium'Tresor'
摘要
Abstract
[Objective]To analyze the expression pattern of the phenylcoumaran benzylic ether reductase gene LaPCBER1 during bulb development in Lilium'Tresor',and to investigate its effects on bulb enlargement as well as its role in regulating lignin content during this process.[Method]By analyzing transcriptomic data from the bulb enlargement process of Lilium'Tresor',a differentially expressed gene annotated as PCBER1 was screened.The LaPCBER1 gene was cloned using cDNA from Lilium'Tresor'bulbs as the template,and the physicochemical properties of the encoded protein were analyze using bioinformatics software.The expression patterns of LaPCBER1 in different tissues and different developmental stages of Lilium'Tresor'were detected by quantitative real-time PCR(qRT-PCR).The effects of LaPCBER1 on bulb enlargement were investigated using transient overexpression techniques,and the lignin content of lily bulbs at different stages and following overexpression was measured using assay kits.[Result]The full-length DNA sequenceof LaPCBER1 is 930 bp,encoding 309 amino acids.The protein is predicted to have 51 phosphorylation sites and is characterized as a non-transmembrane,unstable,hydrophilic protein.qRT-PCR results showed that LaPCBER1 was highly expressed in the stem,with expression peaking during the S4 stage(rapid expansion stage)of bulb development.Following transient overexpression of LaPCBER1,the lignin content in transgenic bulbs increased by 49.7%compared to the vector.The fresh weight,dry weight,and relative growth rates of circumference of the bulbs increased by 29.0%,29.7%,and 37.2 percentage points,respectively;Conversely,after silencing LaPCBER1,the fresh weight,dry weight,and relative growth rates of circumference of the bulbs decreased by 35.9%,38.7%,and 32.2 percentage points,respectively,and the lignin content decreased by 24.3%.[Conclusion]Expression pattern and functional analysis of LaPCBER1 suggest that this gene is highly expressed during the early stages of bulb development and promotes lignin accumulation and bulb expansion.It is therefore hypothesized that LaPCBER1 is involved in the bulb expansion process of Lilium'Tresor'and plays a positive regulatory role in this process.关键词
'Tresor'百合/LaPCBER1 基因/鳞茎膨大/木质素/表达分析/功能分析Key words
Lilium'Tresor'/LaPCBER1 gene/bulbs expansion/lignin/expression analysis/functional analysis分类
农业科技引用本文复制引用
金梦竹,王梦迪,陈妍竹,马艳芳,李青源,康伟丽,潘文强,于晓南,杜运鹏..LaPCBER1基因在'Tresor'百合鳞茎发育过程中的功能解析[J].广东农业科学,2026,53(2):67-79,13.基金项目
国家重点研发计划项目(2024YFD1600902) （2024YFD1600902）
北京市农林科学院科技创新能力建设专项(2026-2027) （2026-2027）
北京市农林科学院优秀青年基金(YKPY2026017) （YKPY2026017）
北京市农林科学院优秀青年科学家培养计划项目(YXQN202303-C) （YXQN202303-C）
