广东农业科学2026，Vol.53Issue(2)：80-89,10.DOI:10.16768/j.issn.1004-874X.2026.02.006
不同蝴蝶兰品种抗高温胁迫性能比较及生理机制研究
Comparative Study on High-temperature Stress Resistance and Physiological Mechanisms Among Different Phalaenopsis Varieties
摘要
Abstract
[Objective]High temperatures during e-commerce storage and transportation may adversely affect the commercial quality of Phalaenopsis orchids and restrict the sustainable development of the industry.This study aimed to preliminarily investigate the response characteristics and resistance mechanisms of different Phalaenopsis cultivars under high-temperature stress,and to identify key indicators associated with heat tolerance,thereby providing references for heat-tolerant cultivar selection and postharvest preservation technologies.[Method]Four Phalaenopsis cultivars,'Ruyu','Yuhong','Xiaozhuangshi',and'Xiaoheidie',were used as experimental materials.A simulated high-temperature environment for e-commerce transportation(40℃day/30℃night)was applied for 72 h,with a normal temperature condition(28℃day/22℃night)as the control.Morphological and anatomical traits,ethylene metabolism,and redox homeostasis were analyzed.The heat injury index,floral organ structure,contents of ACC synthase(ACCS)and ACC oxidase(ACO),activities of superoxide dismutase(SOD)and peroxidase(POD)were measured to compare cultivar differences.[Result]The heat tolerance of the four cultivars ranked as'Xiaoheidie'(heat injury grade 1)>'Xiaozhuangshi'(grade 2)>'Ruyu'='Yuhong'(grade 4).The first two cultivars exhibited relatively mild symptoms,such as slight peduncle drooping or mild wilting of terminal flowers,and recovered within 24 h under normal temperature.In contrast,the latter two cultivars showed obvious flower wilting and bud abortion,resulting in substantial loss of ornamental quality.'Xiaoheidie'and'Xiaozhuangshi'possessed petals approximately 0.7 mm thick with a semi-waxy layer and peduncles 4.6-5.0 mm in diameter with higher lignification,indicating structural advantages.In contrast,the weakly heat-tolerant cultivars had thinner petals(0.38-0.50 mm),lacked a waxy layer,displayed loosely arranged cells,and had thinner peduncles with lower lignification.Under high-temperature stress,'Xiaoheidie'inbibited ACO accumulation,whereas'Xiaozhuangshi'upregulated ACCS content to accumulate ACC.Weakly tolerant cultivars exhibited abnormal or unresponsive enzyme contents,suggesting that the ethylene pathway may not be the key factor determining heat tolerance.No significant changes in SOD activity were observed among the four cultivars.However,POD activity in the weakly heat-tolerant cultivars decreased by 52%and 42%,respectively,compared with the control,whereas the basal POD activity in heat-tolerant cultivars was 2-3 times higher.The basal anthocyanin content in'Xiaoheidie'reached 0.595 mg/g(FW)and decreased by 28%under high temperature,suggesting a compensatory antioxidant role.Malondialdehyde(MDA)increased significantly only in'Ruyu'(by 7%),indicating that it may not be a sensitive indicator for screening Phalaenopsis under the simulated high-temperature transportation conditions used in this study.[Conclusion]Significant differences in heat tolerance exist among Phalaenopsis cultivars.'Xiaoheidie'and'Xiaozhuangshi'exhibited stronger heat tolerance.The adaptation of Phalaenopsis to high-temperature stress during storage and transportation may depend on the synergistic effects of morphological structures and physiological regulation.In particular,petal waxy structure,peduncle lignification,POD activity,ACO content,and anthocyanin content were closely associated with heat tolerance and may serve as important reference indicators for screening heat-tolerant Phalaenopsis cultivars.关键词
蝴蝶兰/高温储运/耐热性/形态结构/乙烯代谢/抗氧化酶/花朵衰老Key words
Phalaenopsis/high-temperature transportation/heat tolerance/morphological structure/ethylene metabolism/antioxidant enzymes/flower senescence分类
农业科技引用本文复制引用
贾茜婷,吕复兵,周武涛,肖文芳,曾燕萍,陈和明,沈友福,叶广英,陈启照,魏鑫钰..不同蝴蝶兰品种抗高温胁迫性能比较及生理机制研究[J].广东农业科学,2026,53(2):80-89,10.基金项目
广东省科技计划项目(2024B1212060012) （2024B1212060012）
广东省农业科学院协同创新中心项目(XTXM202201) （XTXM202201）
广东省科技成果入县达镇促进城乡区域协调发展专项(2025B0202010019) （2025B0202010019）
广东省农业种质资源库圃、畜禽遗传资源保种场运行维护与畜禽核心育种场生产性能测定项目(NJTG20250716) （NJTG20250716）
