高师理科学刊2026，Vol.46Issue(3)：54-61,8.DOI:10.3969/j.issn.1007-9831.2026.03.008
过表达砂藓RcEXG1基因改变转基因拟南芥耐旱性和耐高温性
Overexpression of the RcEXG1 gene from Racomitrium canescens alters drought and high-temperature tolerance in transgenic Arabidopsis thaliana
摘要
Abstract
Exoglucanase(EXG)can participate in the process of plant cell wall remodeling under drought stress by hydrolyzing cellobiose from the ends of cellulose chains.Racomitrium canescens exhibits remarkable dehydration tolerance and high-temperature tolerance,many genes had been discovered from R.canescens and verified related to plant drought and high-temperature tolerance.An exoglucanase gene,RcEXG1,was selected which was differentially expressed during dehydration,rehydration,and high-temperature treatments in R.canescens,to investigate and validate its functions in drought and high-temperature tolerance,aiming to provide a theoretical reference and candidate genes for understanding the stress resistance mechanisms in R.canescens and breeding stress-resistant crops.Used quantitative real-time PCR to detect the expression changes of RcEXG1 during dehydration and high-temperature stress in R.canescens,cloned the coding sequence of RcEXG1 from R.canescens then constructed the plant overexpression vector,in order to acquire RcEXG1 overexpression transgenic Arabidopsis thaliana lines,to analyze plant phenotypes after drought and high-temperature stress treatments.Expression analysis results indicated that RcEXG1 responds to both dehydration and high-temperature stress in R.canescens.The coding sequence of RcEXG1 was successfully cloned and multiple RcEXG1 overexpression transgenic A.thaliana lines were acquired.Under simulated drought stress induced by 20%PEG-6000,the RcEXG1 overexpression transgenic plants exhibited stronger drought tolerance compared to wild-type plants,and the level of drought resistance was positively correlated with the expression level of RcEXG1 in the transgenic plants.However,RcEXG1 overexpression transgenic A.thaliana performed weaker tolerance than the wild-type plants under 45℃high-temperature stress.The results revealed that while overexpression of RcEXG1 improves drought tolerance in transgenic A.thaliana,but concurrently reduces its high-temperature tolerance.关键词
RcEXG1/砂藓/外切葡聚糖酶基因/干旱胁迫/高温胁迫/抗性鉴定Key words
RcEXG1/Racomitrium canescens/exoglucanase gene/drought stress/high-temperature stress/resistance identification分类
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林祎,王晶晶,李婧,孙铭钰,沙伟,张梅娟,彭疑芳,马天意..过表达砂藓RcEXG1基因改变转基因拟南芥耐旱性和耐高温性[J].高师理科学刊,2026,46(3):54-61,8.基金项目
黑龙江省省属高等学校基本科研业务费科研项目(145409452) （145409452）
