广东药科大学学报2026，Vol.42Issue(2)：10-17,8.DOI:10.16809/j.cnki.2096-3653.2025123001
铁铈双金属有机框架的合成及其酶催化抗菌性能研究
Synthesis and enzyme-catalytic antibacterial properties of iron-cerium bimetallic organic frameworks
摘要
Abstract
Objective To provide experimental evidence and theoretical basis for their applications in the antibacterial field,iron-cerium bimetallic organic frameworks(FeCeMOF)were prepared.It's enzyme-catalytic antibacterial properties were systematically investigated.Methods FeCe-MOF was prepared by a one-pot method.The material was characterized by scanning electron microscopy,X-ray diffraction,X-ray photoelectron spectroscopy and other techniques to verify the distribution of Fe,Ce and related elements in the organic ligand,as well as the structural characteristics of the material.The peroxidase-like(POD),catalase-like(CAT)and glutathione peroxidase-like(GPx)activities,together with POD-like enzymatic kinetics,were systematically investigated to evaluate the nanozyme catalytic activity of FeCe-MOF.Using Methicillin-resistant Staphylococcus aureus(MRSA)and Escherichia coli(E.coli)as test strains,in vitro antibacterial experiments were carried out via the plate colony counting method and live/dead fluorescence staining,to study the antibacterial performance and effect of FeCe-MOF.Results Elemental mapping analysis confirmed the successful synthesis of FeCe-MOF,with Fe,Ce,and elements related to the organic ligands showing uniform distribution within the material.Nanozyme catalytic activity tests demonstrated that FeCe-MOF possesses the ability to generate reactive oxygen species(ROS)and can promote ROS accumulation by consuming intracellular glutathione(GSH)in bacteria.Elevated intracellular ROS levels induce a series of oxidative stress responses in bacteria,thereby exerting antibacterial effects.In vitro antibacterial experiments revealed that FeCe-MOF exhibits excellent antibacterial activity against MRSA and E.coli.Conclusion FeCe-MOF exerts bactericidal effects by generating reactive oxygen species(ROS)through its nanozyme activity.Thus,it holds great promise as a novel antibacterial material.关键词
感染性伤口/金属有机框架(MOF)/模拟酶活性/抗菌活性/活性氧Key words
infected wound/metal-organic frameworks(MOF)/nanozyme activity/antibacterial activity/reactive oxygen species分类
医药卫生引用本文复制引用
方浩岚,张雨露,陈思茵,孙端平,由天辉..铁铈双金属有机框架的合成及其酶催化抗菌性能研究[J].广东药科大学学报,2026,42(2):10-17,8.基金项目
广东省基础与应用基础研究基金自然科学基金项目(2024A1515011385) （2024A1515011385）
