广东药科大学学报2026，Vol.42Issue(2)：125-134,10.DOI:10.16809/j.cnki.2096-3653.2026010502
美洲大蠊肠道链霉菌潜在新种G30的鉴定及全基因组分析
Identification and whole-genome analysis of G30,a potential new species of Streptomyces from the gut of the Periplaneta americana
摘要
Abstract
Objective To isolate and characterize a potential novel species of Streptomyces,designated G30,from the gut of Periplaneta americana and evaluate its secondary metabolic potential through polyphasic taxonomy and whole-genome analysis.Methods Bacterial isolation was performed using the dilution plating method.The isolated strain was subsequently identified through polyphasic taxonomy,which included 16S rRNA gene sequencing,morphological observation,and physiological and biochemical characterization.The antitumor activity of its crude extracts against MCF-7,A549,and HepG2 cells was evaluated using the CCK-8 assay.Additionally,whole-genome sequencing was carried out on the Nanopore platform,and the biosynthetic gene clusters(BGCs)were analyzed for novelty with antiSMASH 7.0.Results Strain G30 exhibited a 16S rRNA gene sequence similarity of 98.88%with the type strain Streptomyces physcomitrii LD120.However,significant differences were observed in carbon source utilization,biochemical reactions,and growth conditions,which supported its status as a potential novel species within the genus Streptomyces.The ethyl acetate crude extract of strain G30 demonstrated cytotoxic activity against MCF-7,A549,and HepG-2 cells,with the strongest inhibitory effect observed on MCF-7 cells(IC50=64.16 μg/mL).Genomic analysis identified a total of 33 BGCs.Among these,gene clusters with homology below 30%account for 30.3%of the total,and 18.2%were identified as orphan clusters,indicating a high degree of novelty.These BGCs were predicted to encode the synthesis of potentially antitumor compounds such as murayaquinone,chrysomycin,and ilamycin.Conclusion Strain G30 represents a potential novel species of Streptomyces with highly novel secondary metabolic potential,serving as an important microbial resource for the discovery of antitumor lead compounds.关键词
美洲大蠊/链霉菌/全基因组测序/多相分类鉴定/抗肿瘤活性Key words
Periplaneta americana/streptomyces/whole-genome sequencing/polyphasic taxonomic identification/antitumor activity分类
医药卫生引用本文复制引用
王丽君,温思浩,张翔,郑文娜,陈志宇,刘文彬,金小宝..美洲大蠊肠道链霉菌潜在新种G30的鉴定及全基因组分析[J].广东药科大学学报,2026,42(2):125-134,10.基金项目
广东省普通高校特色创新项目(2025KTSCX055) （2025KTSCX055）
广东省医学科学技术研究基金资助项目(A2024478) （A2024478）
广州市科技计划项目(2025A04J3623) （2025A04J3623）
