广西植物2026，Vol.46Issue(3)：455-464,10.DOI:10.11931/guihaia.gxzw202505036
高山锥叶的成分分离鉴定及其胰脂肪酶抑制活性研究
Isolation and identification of constituents from the leaves of Castanopsis delavayi and their inhibitory activity against pancreatic lipase
摘要
Abstract
A phytochemical investigation of the leaves of Castanopsis delavayi was conducted to explore its constituent compounds and evaluate their inhibitory activity against pancreatic lipase.The 80％methanol extract of the leaves was sequentially purified using multiple chromatographic columns,including Sephadex LH-20,MCI gel CHP 20P,Diaion HP20SS,and Toyopearl HW-40F.The structure of isolated compounds was identified by pop analysis(NMR,MS)in comparison with literature data.An in vitro screening model for pancreatic lipase inhibitory activity was established utilizing the 4-methylumbelliferyl oleate(4-MUO)fluorescence assay,with orlistat serving as the positive control(IC50=0.001 4 mmol·L-1).The results were as follows:(1)Nineteen compounds were successfully isolated and structurally characterized from the 80％methanol extract,namely gallic acid(1),syringic acid(2),(Z)-3,5-dihydroxycinnamic acid(3),(E)-3,5-dihydroxycinnamic acid(4),dehydrodigallic acid(5),3-O-β-D-(6'-O-galloyl)-glucopyranoside(6),gentisic acid-5-O-β-D-(6'-O-galloyl)-glucopyranoside(7),macarangioside E(8),chesnatin(9),theogallin(10),3-O-p-coumaroylquinic acid(11),5-O-caffeoylquinic acid(12),methyl chlorogenate(13),quercetin(14),myricetin-3-O-β-D-glucopyranoside(15),quercetin-3-O-β-D-galactoside(16),quercetin-3-O-glucuronopyranoside(17),quercetin-3-O-β-D-glucuronide-6"-methyl ester(18),and quercetin 3-O-(6"-O-galloyl)-β-D-galactopyranoside(hyperin 6"-gallate)(19).(2)Twelve compounds exhibiting differential inhibitory effects against pancreatic lipase,and compounds 6,7,12,and 17 demonstrated significant inhibitory activity.All compounds were isolated from C.delavayi for the first time,providing a theoretical basis for further research on its lipid-lowering effects.关键词
高山锥/化学成分/分离鉴定/胰脂肪酶/抑制活性Key words
Castanopsis delavayi/chemical constituents/isolation and identification/pancreatic lipase/inhibitory activity分类
农业科技引用本文复制引用
袁红静,庞闹,王亚凤,何瑞杰,刘章彬,李桂勤,黄永林..高山锥叶的成分分离鉴定及其胰脂肪酶抑制活性研究[J].广西植物,2026,46(3):455-464,10.基金项目
广西自然科学基金(2025GXNSFBA069156) （2025GXNSFBA069156）
广西植物功能物质与资源持续利用重点实验室主任基金(ZRJJ2023-8). （ZRJJ2023-8）
