广西植物2026，Vol.46Issue(3)：465-478,14.DOI:10.11931/guihaia.gxzw202504021
抗金黄色葡萄球菌复方精油的药效物质与初步作用机制
Pharmacological substances and preliminary action mechanism of compound essential oil against Staphylococcus aureus
摘要
Abstract
In order to elucidate the pharmacodynamic material basis and mechanisms of underlying the anti-bacterial efficacy of compound essential oils against Staphylococcus aureus,this study employed steam distillation method to prepare single essential oils of Artemisia argyi,Mentha spicata and Origanum vulgare,and two compound essential oils(Compound essential oil 1 and Compound Essential Oil 2);gas chromatography-mass spectrometry(GC-MS)analysis was performed to identify the active constituents of the essential oils exhibiting anti-Staphylococcus aureus activity;the filter paper diffusion assay was utilized to evaluate the anti-bacterial activity of each essential oil against Staphylococcus aureus;network pharmacology and molecular docking simulations were integrated to investigate the potential anti-bacterial mechanism of the most bioactive compound essential oil(Compound Essential Oil 2).The results were as follows:(1)Compound essential oil 2 comprised 41 chemical constituents,with its primary bioactive constituents including thymol(29.33％)and carvacrol(9.13％).(2)The order of anti-bacterial efficacy among single and compound essential oils was Compound Essential Oil 2[(40.18±1.67)mm]＞Origano vulgare essential oil[(38.64±1.47)mm]＞Compound essential oil 1[(24.87±0.85)mm]＞Artemisia argyi essential oil[(13.12±0.56)mm]＞Mentha spicata essential oil[(11.28±0.75)mm].Compound Essential Oil 2 exhibited the strongest inhibitory effect against Staphylococcus aureus.(3)Compound Essential Oil 2 exerted inhibition of Staphylococcus aureus through multi-target synergistic effects,modulating core protein targets including SRC and MAPK3,and regulating signaling pathways of notably neutrophil extracellular trap formation,drug resistance of epidermal growth factor receptor tyrosine kinase inhibitor(EGFR-TKI)and so on.Several bioactive constituents showed favorable binding energies with core targets.These collective findings demonstrate that Compound Essential Oil 2 is a novel plant-derived anti-Staphylococcus aureus candidate,and its unique chemical constituents,multi-target and multi-pathway synergistic mechanism provide important basis for the in-depth development and application as a natural anti-bacterial agent.关键词
复方精油/抗金黄色葡萄球菌/药效物质基础/作用机制/质谱分析Key words
compound essential oil/against Staphylococcus aureus/pharmacological substance basis/action mechanism/mass spectrometry analysis分类
农业科技引用本文复制引用
刘俊娜,金成,卢清煜,张晓燕,徐志,周威..抗金黄色葡萄球菌复方精油的药效物质与初步作用机制[J].广西植物,2026,46(3):465-478,14.基金项目
贵州省教育厅高校重点领域项目(KY字[2021]048) （KY字[2021]048）
贵州省卫生健康委科学技术基金(gzwkj2021-451) （gzwkj2021-451）
贵州医科大学大学生创新创业训练计划项目(2025-YX30). （2025-YX30）
