广西植物2026，Vol.46Issue(3)：507-520,14.DOI:10.11931/guihaia.gxzw202505035
广西莪术不同组织内生真菌多样性、环境适应性与药用成分积累的关系
Diversity,environmental adaptation,and medicinal component accumulation of endophytic fungi in different tissues of Curcuma kwangsiensis
摘要
Abstract
Curcuma kwangsiensis is a Daodi medicinal material of Curcuma in Zingiberaceae,yet the mechanism underlying its quality formation remains unclear.To elucidate the interaction relationships between endophytic fungal communities,soil environments,and medicinal material quality,and to reveal the microbiological basis of its geographical authenticity.C.kwangsiensis samples were collected from three major production areas in Qinzhou City(Pubei County,Lingshan County,and Qinnan District).Using ITS high-throughput sequencing and chemical analysis methods,the endophytic fungal community structures in five tissues(root,rhizome,stem,leaf,and flower)were systematically studied,and the physicochemical properties of the rhizosphere soil and quality markers of the medicinal materials were analyzed.Redundancy analysis(RDA)and correlation analysis were employed to explore the relationships between microbial communities,environmental factors,and medicinal material quality.The results were as follows:(1)A total of 2 357 operational taxonomic units(OTUs)(902 species of fungi)were obtained from 45 samples;the distribution of OTUs among the three regions differed significantly,with only 19.4％being common core OTUs across regions;leaf tissues had the highest number of total OTUs(1 205)and unique OTUs(415),while the shared OTUs among the five tissue types accounted for only 6.9％.(2)Above-ground tissues(leaf and flower)exhibited significantly higher fungal richness(ACE and Chao 1 indexes)and diversity(Shannon index)than below-ground tissues(root and rhizome).Additionally,NMDS analysis revealed distinct differences in community structures between above-ground and below-ground tissues.(3)The core genus analysis indicated that the below-ground tissues were dominated by taxa such as unclassified-c—Sordariomycetes and wood-decomposing fungi,while the above-ground tissues were characterized by dominant genera like Cladosporium and Alternaria.The stem tissues displayed transitional characteristics in their fungal composition.(4)The RDA demonstrated that soil pH,organic matter,and nutrients(nitrogen,phosphorus,and potassium)significantly influenced the fungal community composition;rhizome samples from Pubei County showed the highest olibanum alcohol-soluble extract content(ASEC).Notably,the rhizome fungal community was closely associated with ASEC levels,and unclassified-c—Sordariomycetes in rhizomes showed a positive correlation with ASEC,whereas Acremonium exhibited a negative correlation with ASEC.The above results indicate that the endophytic fungal community in Curcuma kwangsiensis exhibits significant regional specificity and tissue niche differentiation characteristic.Specific fungal taxa may participate in the accumulation of medicinal compounds through microenvironmental regulation,providing new insights into the microbial mechanisms underlying the formation of Daodi medicinal material quality.关键词
广西莪术/内生真菌/高通量测序/组织特异性/土壤理化性质Key words
Curcuma kwangsiensis/endophytic fungi/high-throughput sequencing/tissue specificity/soil physicochemical properties分类
农业科技引用本文复制引用
龙艳艳,黄子怡,曾凤花,张艳,谢飞,赵惠莉,谢玲..广西莪术不同组织内生真菌多样性、环境适应性与药用成分积累的关系[J].广西植物,2026,46(3):507-520,14.基金项目
广西自然科学基金(2022GXNSFBA035551) （2022GXNSFBA035551）
广西重点研发计划项目(桂科AB22035012) （桂科AB22035012）
广西农业科学院基本科研业务专项(桂农科2021YT065,桂农科2026YT134). （桂农科2021YT065,桂农科2026YT134）
