广西植物2026，Vol.46Issue(3)：521-533,13.DOI:10.11931/guihaia.gxzw202504023
内生拟茎点霉菌剂对两面针生长和土壤微生态的影响
Effects of endophytic Phomopsis sp.microbial agent on growth and soil microecology of Zanthoxylum nitidum
摘要
Abstract
To investigate the effects of the endophytic Phomopsis Y118 agent on the growth of Zanthoxylum nitidum and soil microecology,this study measured and analyzed the biomass,active ingredient content,soil physicochemical properties,soil enzyme activities and soil microbial composition structure using Z.nitidum as the experimental material.A pot experiment was conducted with five dosage levels of the microbial agent.The results were as follows:(1)The application of Y118 agent significantly increased the biomass of Z.nitidum.Compared to the control,the leaf fresh weight,leaf dry weight,root fresh weight and root dry weight achieved their maximum values under 25 g·plant-1,showing increases of 118.4％,73.5％,192.2％and 89.4％,respectively.(2)The application of Y118 agent improved the accumulation of active ingredients.The contents of nitidine chloride and chelerythrine reached their peak levels at the dosage of 35 g·plant-1,increasing by 160.9％and 53.8％compared to the control,respectively.(3)The application of Y118 agent modulated soil fertility to varying degrees.At a dose of 25 g·plant-1,the contents of alkaline nitrogen and available phosphorus reached their peak levels,increasing by 13.6％and 17.9％compared to the control,respectively.(4)The application of Y118 agent increased the activity of soil enzyme to varying degrees.At a dose of 25 g·plant-1,the activities of sucrase and catalase in the soil reached the maximum values,which were 42.9％and 13.4％higher than the control,respectively.(5)The application of Y118 agent regulated the soil microbiome structure to some extent,with Saitozyma,Pseudomonas and Aspergillus emerging as the predominant genera.In conclusion,Y118 agent promotes the growth and accumulation of active ingredients in Zanthoxylum nitidum,which may be related to improving soil fertility and enzyme activity,and influencing the soil micro-ecological environment.The results of this study provide a scientific foundation for the green cultivation and scientific fertilization of Z.nitidum.关键词
两面针/内生拟茎点霉/微生物菌剂/促生作用/土壤微生态Key words
Zanthoxylum nitidum/endophytic Phomopsis sp./microbial agent/promoting growth/soil microecology分类
农业科技引用本文复制引用
伍春凤,罗焯方,梁子宁,谢泽碧,张瑞蓉,申骁烈..内生拟茎点霉菌剂对两面针生长和土壤微生态的影响[J].广西植物,2026,46(3):521-533,13.基金项目
广西自然科学基金(2024GXNSFAA010350) （2024GXNSFAA010350）
广西壮族自治区科技厅项目(2020GXNSFAA259043) （2020GXNSFAA259043）
广西壮瑶药重点实验室项目(桂科基字[2014]32号) （桂科基字[2014]32号）
国家中医药管理局高水平中医药重点学科建设项目(zyyzdxk-2023165) （zyyzdxk-2023165）
壮瑶药协同创新中心项目(桂教科研[2013]20号) （桂教科研[2013]20号）
大学生创新创业训练计划项目(S202410600137). （S202410600137）
