高原农业2026，Vol.10Issue(2)：206-216,11.DOI:10.19707/j.cnki.jpa.2026.02.008
四种提取青稞种子DNA方法的比较研究
Comparative Study of Four Methods for DNA Extraction from Highland Barley Seeds
摘要
Abstract
To verify the applicability of a self-developed maize single-seed endosperm DNA extraction method that preserves seed viability(referred to as the"patented method")in barley seeds,this study used different barley varieties as materials.DNA was extracted from the seeds by the traditional CTAB method,the modified CTAB method,the alkaline boiling method,and the patented method,respectively.The concentration and purity of the obtained DNA were detected by agarose gel electrophoresis and ultraviolet spectrophotometry.Furthermore,wiht the extracted DNA as a template,conventional PCR,multiplex PCR,and real-time fluorescent quantitative PCR were performed to amplify five genes related to starch synthesis,drought resistance,and disease resistance.The results showed that the DNA extracted from barley seeds by the patented method had a high concentration and good purity and exhibited excellent amplification efficiency in the target gene amplifications,fully meeting the requirements for subsequent molecular biology experiments.Meanwhile,seeds that had undergone partial endosperm excision could still germinate and grow normally and could be used for subsequent related research.This study expands the application range of the patented method to other crop types and provides a high-quality single-seed endosperm DNA extraction method that preserves seed viability for molecular biology research in barley.关键词
青稞品种/DNA提取方法/CTAB法/碱煮法Key words
Highland barley varieties/DNA extraction method/CTAB method/Alkaline boiling method分类
农业科技引用本文复制引用
茆倩倩,李展凤,刘凯鑫,王江昊,刘安邦,郭新梅..四种提取青稞种子DNA方法的比较研究[J].高原农业,2026,10(2):206-216,11.基金项目
山东省重点研发计划(农业良种工程)玉米两高三抗绿色相关基因的克隆与种质创新及品种选育项目(2022LZGC0063) （农业良种工程）
山东省技术创新引导计划青稞高活性浓浆绿色高效制备技术与高端产品创制项目(YDZX2024004). （YDZX2024004）
