贵州农业科学2026，Vol.54Issue(4)：40-46,7.DOI:10.3969/j.issn.1001-3601.2026.04.005
生姜组培苗后代群体的性状分析
Analysis on Traits of Offspring Population of Ginger Tissue Culture Seedlings
摘要
Abstract
[Objective]The variation mechanism of ginger tissue culture seedlings was investigated to effectively select the mutant seedlings for reducing the cost in subculture,transplanting and later management and lay the foundation for innovation of ginger germplasm resources.[Method]The differences between mutant and normal tissue culture plants were analyzed from the angles of phenotypic traits,cytology and molecular level.[Result]The mutant plants from M'3 generation showed dwarf,more branch number and lower rhizome weight,and no significant difference in fresh leaf weight compared with the normal plant.A total of 98 bands with a polymorphism ratio of 77.36%and polymorphism information content(PIC)of 0.646 5-0.890 0 were amplified by 12 pairs of SRAP primers.The genetic identity and genetic distance of four ginger populations(tissue culture seedling,breeder's seed,original seed and production seed)were 0.674 7-0.861 0 and 0.149 6-0.393 5 separately.The chromosome number of root tip cells in normal(M3)and mutant(M'3)plants both was 22(2n=2x=22).[Conclusion]There is a significant variation in phenotypic characteristics of plants from ginger production seed and no variation in chromosome number of plants from ginger production seed.At the molecular level,there is an extensive somatic cell variation in the offspring of ginger tissue culture seedlings.关键词
生姜/突变植株/表型性状/分子标记/染色体/体细胞Key words
ginger/mutant/phenotypic trait/molecular marker/chromosome/somatic cell分类
农业科技引用本文复制引用
李晋华,王少铭,罗莉斯,侯颖辉,冷家归,汪志燚,魏桂民,李德文..生姜组培苗后代群体的性状分析[J].贵州农业科学,2026,54(4):40-46,7.基金项目
贵州省农业科学院青年基金项目(黔农科院青年基金[2022]27号) （黔农科院青年基金[2022]27号）
贵州省科技支撑计划项目(黔科合支撑[2021]一般260),(黔科合支撑[2022]一般120) （黔科合支撑[2021]一般260）
贵州省农业科学院专项(黔农科种质资源[2023]16号) （黔农科种质资源[2023]16号）
