贵州农业科学2026，Vol.54Issue(4)：74-85,12.DOI:10.3969/j.issn.1001-3601.2026.04.009
草地贪夜蛾1龄虫取食胁迫对高粱幼苗叶片和根部转录组的影响
Effects of 1st-instar Spodoptera frugiperda Feeding Stress on Leaf and Root Transcriptomes of Sorghum Seedlings
摘要
Abstract
[Objective]The effects of 1st-instar Spodoptera frugiperda feeding on leaf and root transcriptomes of sorghum seedlings were explored to provide a theoretical basis for illustrating the molecular mechanism in response of sorghum seedlings to S.frugiperda feeding and breeding insect-resistant sorghum varieties.[Method]The leaf and root samples of Hongliangfeng 1(a sorghum variety)seedlings fed by 1st-instar S.frugiperda for 9 h(TR)were analyzed for transcriptome sequencing by taking normal Hongliangfeng 1 seedlings as CK.[Result]A total of 1 509 differentially expressed genes(DEGs)of 1 202 up-regulated and 307 down-regulated DEGs were identified in leaves,and 2 128 DEGs of 1 493 up-regulated and 635 down-regulated DEGs were identified in roots,which showed that DEGs were of tissue-specificity.The up-regulated genes in leaves were primarily involved in defense-related pathways of secondary metabolite synthesis,fatty acid metabolism,jasmonic acid biosynthesis,and so on,and the down-regulated genes in leaves were associated with DNA repair and reproduction.The up-regulated genes in roots were enriched in pathways of phenylpropanoid biosynthesis,cutin and waxiness synthesis,and phytohormone signaling transduction,and the down-regulated genes were related to growth and transport process.[Conclusion]The leaves of sorghum seedlings respond to S.frugiperda feeding stress by activating defense metabolic pathways and preferentially allocating defense resource,while the roots involves in defense by signal reception,synergistic response,and resource reallocation.关键词
草地贪夜蛾/高粱/幼苗/转录组/取食/叶片/根部Key words
Spodoptera frugiperda/sorghum/seedling/transcriptome/feeding/leaf/root分类
农业科技引用本文复制引用
沈佳奇,陈满静,邵明波..草地贪夜蛾1龄虫取食胁迫对高粱幼苗叶片和根部转录组的影响[J].贵州农业科学,2026,54(4):74-85,12.基金项目
贵州省基层农业技术推广补贴项目(仁怀基地示范[2025]01) （仁怀基地示范[2025]01）
中央引导地方科技发展资金"酒糯高粱新品种'红粱丰1号'良种繁育及配套栽培技术集成与示范"(黔科中引地[2022]4045) （黔科中引地[2022]4045）
贵州省农业科学院科技创新项目"贵州糯高粱小曲清香型白酒酿造关键技术研究与示范"(黔农科院科技创新[2022]11号) （黔农科院科技创新[2022]11号）
