甘蔗糖业2026，Vol.55Issue(2)：11-19,9.DOI:10.3969/j.issn.1005-9695.2026.02.002
甘蔗黄叶病毒CP基因质粒标准样品的研制
Development of a Plasmid Standard Sample of Sugarcane Yellow Leaf Virus CP Gene
摘要
Abstract
To address the lack of standard samples for sugarcane yellow leaf virus(SCYLV)nucleic acid detection,this study constructed a recombinant plasmid(pMD19-SCYLV-CP)with the SCYLV coat protein gene,prepared a plasmid DNA standard sample,and verified its characteristics.The results showed that the certified reference material exhibited good homogeneity(F=1.20＜F0.05=2.22).Stability assessments revealed that the material remained stable under simulated transport conditions(4-37℃for 14 days),long-term storage(-20℃for 24 months),and repeated freeze-thaw cycles(25 cycles),with consistent collaborative characterization from 12 labs(all SCYLV-positive).This sample provides the first standardized reference for SCYLV detection in China,supporting scientific testing and quality supervision of virus-free seedlings for SCYLV.关键词
甘蔗黄叶病毒/外壳蛋白基因/实时荧光定量PCR(qPCR)/标准样品Key words
Sugarcane yellow leaf virus/Coat protein gene/Real-time quantitative PCR(qPCR)/Standard sample分类
农业科技引用本文复制引用
毕郑旺,盛裕建,黄美婷,王勤南,孙生仁,高三基..甘蔗黄叶病毒CP基因质粒标准样品的研制[J].甘蔗糖业,2026,55(2):11-19,9.基金项目
国家现代农业糖料产业技术体系专项(CARS-17) （CARS-17）
