甘蔗糖业2026，Vol.55Issue(2)：87-97,11.DOI:10.3969/j.issn.1005-9695.2026.02.009
甘蔗多酚抗氧化活性的DPPH、ABTS和FRAP法测定及测量不确定度评定
Determination of Antioxidant Activity of Sugarcane Polyphenols by DPPH,ABTS and FRAP Methods and Evaluation of Measurement Uncertainty
摘要
Abstract
To determine and evaluate the antioxidant activity of sugarcane polyphenol extracts,establish a multi-index combined determination method applicable to sugarcane polyphenols,and systematically analyze and evaluate the measurement uncertainty of each determination method.Three classical in vitro antioxidant activity assays—the 2,2-diphenyl-1-picrylhydrazyl(DPPH)radical scavenging method,the 2,2ʹ-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)radical scavenging method,and the ferric reducing antioxidant power(FRAP)method—were used to evaluate the antioxidant activity of sugarcane polyphenol extracts,with systematic validation of methodological parameters.All three methods showed excellent linearity,with correlation coefficients R2 greater than 0.999.Precision RSD was less than 2.0％,repeatability RSD was less than 3.5％,and stability was satisfactory.Spike recoveries ranged from 97.4％to 100.5％,and the limits of detection were 1.21,1.05 μg/mL,and 5.50 μmol/L,respectively.The DPPH radical scavenging rate was 78.32％±1.39％(k=2);ABTS radical scavenging rate was 85.47％±1.72％(k=2);and the FRAP value was(456.8±10.6)μmol/g(k=2),all at approximately 95％confidence level.The primary sources of measurement uncertainty were sample solution preparation,absorbance measurement,temperature control,and repeatability.The determination methods are accurate and reliable,and the measurement uncertainty evaluation provides technical support for quality control and standardized testing of sugarcane polyphenol antioxidant activity.关键词
甘蔗多酚/抗氧化活性/测量不确定度/DPPH/ABTS/FRAPKey words
Sugarcane polyphenols/Antioxidant activity/Measurement uncertainty/DPPH/ABTS/FRAP分类
农业科技引用本文复制引用
宁方尧,黄凯,梁勇,温韬..甘蔗多酚抗氧化活性的DPPH、ABTS和FRAP法测定及测量不确定度评定[J].甘蔗糖业,2026,55(2):87-97,11.基金项目
广西教育厅2025 年职业教育教学改革项目(GXGZJG2025A010) （GXGZJG2025A010）
广西工业职业技术学院 2024 年度第一批校级科研项目(GYKY2024001A) （GYKY2024001A）
广西工业职业技术学院 2025 年度第一批校级教科研项目(GYJG2025008A) （GYJG2025008A）
