耕作与栽培2026，Vol.46Issue(3)：13-17,5.
继代培养对马桑菌生长速度和漆酶活力的影响
Effects of Subculture on the Growth Rate and Laccase Activity of Lentinula edodes
摘要
Abstract
To investigate the effects of subculture on the growth and laccase activity of Lentinula edodes(a characteristic edible fungus in Guizhou Province),the mycelial growth characteristics,antagonistic reactions,and laccase activity of the fungus during subculture were studied.The results showed that no antagonistic reaction was observed between any subcul-tured strain and the F1 generation,indicating that genetic variation within the F15 generation could not be determined based solely on phenotypic observation.The daily mycelial growth rate exhibited an overall decreasing trend with increasing sub-culture generations,with the F1 and F7 generations showing the fastest growth,reaching a daily growth rate of 0.95 mm/d.The mycelial density and whiteness near the inoculation point were higher in the F1 to F5 generations compared to other subcultured strains,whereas sectoring was observed in the F13 and F15 generations.Laccase activity was positively pro-moted from F1 to F9 generations,but fluctuated more irregularly after the F9 generation.The highest laccase activity(113.89 U/L)was observed in the F13 generation,which was 1.26 times higher than that of the F1 generation.Considering both mycelial growth and laccase activity,it is recommended that the number of subculture generations should be con-trolled within F5 in the practical spawn production of Lentinula edodes to ensure spawn quality and cultivation safety.关键词
马桑菌/继代培养/拮抗反应/漆酶活力Key words
Lentinula edodes/subculture/antagonistic reaction/laccase activity分类
农业科技引用本文复制引用
李鹏,熊雪,旷紫妃,赵晓朋..继代培养对马桑菌生长速度和漆酶活力的影响[J].耕作与栽培,2026,46(3):13-17,5.基金项目
贵州科学院基金项目"基于区域野生资源的贵州适栽优质香菇新品种选育"(黔科院J字[2023]14号) （黔科院J字[2023]14号）
贵州省科技计划项目"贵州特色马桑菌良种选育及配套高效栽培技术研究"(黔科合支撑[2023]一般052) （黔科合支撑[2023]一般052）
贵州省食用菌科技攻关重点项目"武陵山区野生食用菌人工驯化与试验示范"(黔农菌重点[2024]02) （黔农菌重点[2024]02）
