广州中医药大学学报2026，Vol.43Issue(4)：936-944,9.DOI:10.13359/j.cnki.gzxbtcm.2026.04.016
基于靶向代谢组学探究定坤丹对高龄低预后性不孕症患者卵泡液代谢微环境的调控作用
Investigating the Regulatory Effect of Dingkun Dan on the Metabolic Microenvironment of Follicular Fluid in Elderly Patients with Poor-Prognosis Infertility Based on Targeted Metabolomics
摘要
Abstract
Objective To explore the regulatory effect of Dingkun Dan,a traditional Chinese medicine(TCM)formula for tonifying the kidney and invigorating the spleen,on the metabolic microenvironment of follicular fluid in elderly patients with poor-prognosis infertility.Methods Thirty elderly patients with poor-prognosis infertility undergoing in vitro fertilization-embryo transfer(IVF-ET)at the Affiliated Hospital of Shandong University of Traditional Chinese Medicine from January 2024 to January 2025 were randomly selected.Based on the prior treatment medication,the patients were divided into a Dingkun Dan group(n=15)and a placebo group(n=15).Follicular fluid samples(5 mL)collected on the day of oocyte retrieval were analyzed using UHPLC-QTRAP MS quantitative metabolomics technology.Differential metabolites were screened based on a P-value＜0.05,followed by multivariate statistical analysis and bioinformatic analysis.Results A total of 13 differential metabolites were screened out from the follicular fluid through targeted metabolomics,among which 6 were upregulated and 7 were downregulated.The upregulated metabolites mainly included L-malic acid,5-hydroxy-L-tryptophan,2-hydroxybutyric acid,alpha-hydroxyisobutyric acid,and N-acetyl-L-alanine.The downregulated metabolites mainly included beta-alanine,sarcosine,l-alanine,1H-indole-3-acetamide,pyrrole-2-carboxylic acid,and N-acetyltryptophan.These metabolites primarily belonged to five categories:amino acids,hydroxy acids,indoles,benzene-ring compounds,and pyrroles.Conclusion Dingkun Dan may potentially improve the IVF-ET pregnancy outcomes in elderly patients with poor-prognosis infertility by regulating the metabolic microenvironment of follicular fluid,enhancing antioxidant capacity,and improving oocyte quality.关键词
补肾健脾/定坤丹/高龄/低预后性不孕症/体外受精-胚胎移植/妊娠结局/卵泡液/代谢微环境/靶向代谢组学Key words
tonifying kidney and invigorating spleen/Dingkun Dan/elderly/poor-prognosis infertility/in vitro fertilization-embryo transfer(IVF-EF)/pregnancy outcome/follicular fluid/metabolic microenvironment/targeted metabolomics分类
医药卫生引用本文复制引用
张江琳,高丹丹,孙振高..基于靶向代谢组学探究定坤丹对高龄低预后性不孕症患者卵泡液代谢微环境的调控作用[J].广州中医药大学学报,2026,43(4):936-944,9.基金项目
国家重点研发计划项目(编号:2022YFC3500403) （编号:2022YFC3500403）
国家自然科学基金项目(编号:82274572) （编号:82274572）
山东省重点研发计划项目(编号:2023CXGC010505) （编号:2023CXGC010505）
