复方扶芳藤合剂对黑色素瘤小鼠脾脏CD4+T细胞的调节作用
The regulatory effect of Compound Fufangteng Mixture on CD4+T cells in the spleen of melanoma mice
摘要
Abstract
Objective:To explore the regulatory effect of Compound Fufangfuteng Mixture(CFM)on CD4+T cells in the spleen of melanoma mice.Methods:Randomly divide 50 female C57BL/6 mice into 5 groups:blank group(Blank),tumor model group(B16),high-dose Compound Fufangfuteng Mixture group(CFM-H),medium-dose Compound Fufangfuteng Mixture group(CFM-M),and low-dose Compound Fufangfuteng Mixture group(CFM-L).Pre-treat the mice with pure water or different doses of CFM by gavage for 21 days,followed by subcutaneous injection of B16 cells to establish melanoma model.After 2 weeks,ter-minate the experiment,harvest the mouse spleens,and use flow cytometry to detect the proportion of T cells and their activation,proliferation,apoptosis,inhibition,transcription factor expression and cytokine secretion.Results:CFM had no significant effect on mouse body weight and thymus index,but the medium dose of CFM could increase the spleen index;the medium dose of CFM reduced the proportion of CD4+T cells and increased the proportion of CD8+T cells,with no significant effect on the total T cell proportion;the medium dose of CFM could promote the increase in the number of Th1 and Th2 cells;The medium dose of CFM could promote the expression of CD69 and CD38 on T cells;the high dose of CFM could promote the expression of CD95 on T cells,while the medium and low doses of CFM could promote the expression of PD-1 on T cells;The high dose of CFM could promote the secretion of IL-4,the medium dose of CFM could promote the increase of IL-2,IFN-γ,IL-4,and IL-10,and the low dose of CFM could promote the secretion of TNF-α and IL-4;both high and medium doses of CFM could increase the expres-sion of NF-κB,the medium dose of CFM could decrease the expression of T-bet and GATA-3,and the low dose of CFM could reduce the expression of T-bet.Conclusions:CFM can activate CD4+T cells and promote the differentiation of Th1 and Th2 cell subsets.关键词
复方扶芳藤合剂/黑色素瘤/T细胞Key words
Compound Fufangteng Mixture/Melanoma/T cells分类
医药卫生引用本文复制引用
杨晨一,徐思溢,刘思嘉,刘显,吴展帅,肖健..复方扶芳藤合剂对黑色素瘤小鼠脾脏CD4+T细胞的调节作用[J].海南医科大学学报,2026,32(7):488-501,14.基金项目
This study was supported by the National Natural Science Foundation of China(82460863) （82460863）
Guangxi Natural Science Foundation(2025GXNSFAA069826,2020GXNSFAA297162) （2025GXNSFAA069826,2020GXNSFAA297162）
Guangxi University of Chinese Medicine Youth Research Innovation Team Project(2023TD001) （2023TD001）
Guangxi University of Chinese Medicine High‑level Talent Cultivation Innovation Team Project(2022B006) （2022B006）
Guangxi Natural Science Foundation Youth Science Foundation(2020GXNSFBA159064) （2020GXNSFBA159064）
Guangxi University of Chinese Medicine Undergraduate Innovation and Entrepreneurship Training Program Project(S202310600083,S202310600117) 国家自然科学基金地区科学基金(82460863) （S202310600083,S202310600117）
广西自然科学基金面上项目(2025GXNSFAA069826,2020GXNSFAA297162) （2025GXNSFAA069826,2020GXNSFAA297162）
广西中医药大学青年研究创新团队项目(2023TD001) （2023TD001）
广西中医药大学高层次人才培育创新团队项目(2022B006) （2022B006）
广西自然科学基金青年科学基金(2020GXNSFBA159064) （2020GXNSFBA159064）
广西中医药大学大学生创新创业训练计划项目(S202310600083,S202310600117) （S202310600083,S202310600117）
