海南医科大学学报2026，Vol.32Issue(7)：519-530,539,13.DOI:10.13210/j.cnki.jhmu.20250306.001
生物信息学视角下SPHK2于胃癌中的表达剖析及其潜在诊疗价值探究
The expression analysis of SPHK2 in gastric cancer and its potential diagnostic and therapeutic value from the perspective of bioinformatics
摘要
Abstract
Objective:To investigate the expression of sphingosine kinase 2(SPHK2)in gastric cancer(GC)and its relation-ship with prognosis by bioinformatics and cell experiments,and to evaluate its potential diagnostic and therapeutic value.Methods:The expression of SPHK2 in GC was analyzed by HPA and GEPIA databases,and its correlation with MKI67(Ki-67)expres-sion was tested.Kaplan-Meier Plotter database was used to evaluate the relationship between SPHK2 expression and prognosis of GC patients.The relationship between SPHK2 and tumor microenvironment was explored by UCSC and TIMER2.0 database.The association of SPHK2 expression with immunotherapy was studied based on the Sangerbox database.The cBioPortal and Metascape databases were used to analyze the mutation and enrichment of SPHK2 gene.Immunohistochemical staining(IHC)was used to detect the correlation between SPHK2 expression and clinicopathological features of GC patients.SPHK2 was knocked down by lentivirus in HGC-27 cell line.Western blot was used to detect the expression of each protein.CCK-8 and clone formation experiments were used to investigate cell proliferation.Cell scratch and Transwell chamber experiments were used to evaluate cell migration.Results:The expression of SPHK2 was significantly up-regulated in GC tissues(P<0.05),which was positively correlated with the expression of MKI67(r=0.21,P=1.1×10−5).Survival analysis showed that high expression of SPHK2 in GC was associated with poor overall survival(P=0.000 24)and post-progressive survival(P=1.1×10−6).TIM-ER2.0 and Sangerbox database analysis showed that SPHK2 expression was associated with B cells and CD4+T cells(P<0.05),and could be used as an indicator to evaluate the efficacy of immune checkpoint inhibitors in patients with GC.GO and KEGG en-richment analysis showed that SPHK2 may be involved in multiple GC-related pathways.Cell experiments showed that the prolif-eration and migration of the control group(SPHK2-nc)were better than those of the experimental group(SPHK2-sh),indicating that SPHK2 had a significant effect on the proliferation and migration of GC cells(P<0.05).Conclusions:SPHK2 is highly ex-pressed in GC patients,affects cell proliferation and migration,and is closely related to tumor microenvironment and immune checkpoint genes.It can be used as a potential index to evaluate the diagnosis,prognosis and immunotherapy effect of GC.关键词
胃癌/SPHK2/生物信息学/预后/细胞增殖与迁移/免疫治疗Key words
Gastric cancer/SPHK2/Bioinformatics/Prognosis/Cell proliferation and migration/Immunotherapy分类
医药卫生引用本文复制引用
王睿朋,李婷婷,管佳佳,纪凯,凌潜龙,满文江,常博达,朱冰..生物信息学视角下SPHK2于胃癌中的表达剖析及其潜在诊疗价值探究[J].海南医科大学学报,2026,32(7):519-530,539,13.基金项目
This study was supported by the Natural Science Research Project of Higher Education Institutions in Anhui Province(KJ2021ZD0090) （KJ2021ZD0090）
Graduate Research Innovation Project of Bengbu Medical College(Byycx22090) （Byycx22090）
Provincial Quality Engineering Project for New Era Education in Anhui Province in 2023(2023xscx125) 安徽省高等学校自然科学研究项目(KJ2021ZD0090) （2023xscx125）
蚌埠医学院研究生科研创新项目(Byycx22090) （Byycx22090）
安徽省2023年度新时代育人省级质量工程项目(2023xscx125) （2023xscx125）
