纺织工程学报2026，Vol.4Issue(2)：87-96,10.DOI:10.20150/j.cnki.fzgcxb.2026.02.008
原位改性法制备R Protein A亲和层析膜及对IgG分离纯化性能研究
In-situ modification strategy for fabricating recombinant pro-tein a affinity chromatography membrane and its application in IgG separation
摘要
Abstract
Traditional Protein A affinity chromatography media employ resin bead materials with dense structures,high mass transfer resistance,and low flow rates.To address these limitations,constructed an affinity chromatography mem-brane composed of nanofibers.Ethylene-vinyl alcohol(EVOH)copolymer nanofibers were prepared via melt-blending phase separation,followed by slurry processing and vacuum filtration drying to fabricate the nanofiber membrane(NFM)support.Sodium hydroxide(NaOH)alkaline activation and sodium periodate(NaIO4)oxidation were applied to introduce aldehyde groups(CHO),enabling covalent immobilization of recombinant Protein A onto the membrane sur-face through in-situ reaction,yielding the affinity chromatography membrane(ONFM-3h-R).The effects of oxidant con-centration and reaction time on membrane morphology,chemical properties,hydrophilicity/hydrophobicity,and mechani-cal performance were investigated.Optimal preparation parameters and immunoglobulin G(IgG)separation perfor-mance were systematically evaluated.Results demonstrated that at 0.7 mol/L NaIO4 with 3 h oxidation,the resulting membrane(ONFM-3h)maintained well-preserved porous structures,achieved aldehyde group content of 0.85 mmol/g,exhibited favorable hydrophilicity,and demonstrated tensile stress of 3.62 MPa with 6.43%strain.Recombinant Protein A adsorption reached 8.90 mg/g.Furthermore,the affinity membrane showed an IgG adsorption capacity of 3.65 mg/g,specifically separated IgG from IgG-BSA mixed solutions,and maintained>90%adsorption capacity retention after three reuse cycles.This approach provides a novel strategy for enhancing the efficiency of traditional separation materi-als.关键词
EVOH纳米纤维/重组蛋白A/原位反应/亲和层析膜/IgG分离纯化Key words
EVOH nanofibers/recombinant Protein A/in-situ reaction/affinity chromatography membrane/IgG separa-tion and purification分类
轻工纺织引用本文复制引用
杨栋,周丰,刘轲,李明月,张伊诺,李登科,程盼,朱绍存,朱丽萍,梅涛,向阳..原位改性法制备R Protein A亲和层析膜及对IgG分离纯化性能研究[J].纺织工程学报,2026,4(2):87-96,10.基金项目
国家自然科学基金项目(U23A20585,52273061) （U23A20585,52273061）
湖北省自然科学基金杰出青年项目(2023AFA086) （2023AFA086）
湖北省技术创新计划项目(2024BAB119) （2024BAB119）
武汉市科技计划项目(2023030103010633) （2023030103010633）
