河北医学2026，Vol.32Issue(4)：542-549,8.DOI:10.3969/j.issn.1006-6233.2026.04.03
miR-613调控SphK1/S1P通路对IL-1β诱导的软骨细胞损伤的影响
The Effect of miR-613 on IL-1β-Induced Chondrocyte Injury by Regulating the SphK1/S1P Pathway
摘要
Abstract
Objective:To explore the effect of microRNA-613(miR-613)on interleukin-1β(IL-1β)-induced chondrocyte injury by regulating the sphingosine kinase 1(SphK1)/sphingosine 1-phosphate(S1P)pathway.Methods:Dual luciferase reporter gene assay was used to detect the interaction between miR-613 and SphK1.CHON-001 cells were divided into six groups:Ctrl group(normal culture),IL-1β group(10ng/mL IL-1β induction),NC-mimics group(NC-mimics transfection+IL-1β induction),miR-613-mimics group(miR-613-mimics transfection+IL-1β induction),miR-613-mimics+OE-NC group(miR-613-mimics+OE-NC co-transfection+IL-1β induction),and miR-613-mimics+OE-SphK1 group(miR-613-mimics+OE-SphK1 co-transfection+IL-1β induction).QRT-PCR was used to detect the expression of miR-613,SphK1,and S1P mRNA in CHON-001 cells.MTT and EdU staining were used to measure CHON-001 cell proliferation.Flow cytometry and TUNEL staining were performed to measure CHON-001 cell apopto-sis.An ELISA kit was used to measure the expression of IL-6 and TNF-α in CHON-001 cells.Moreover,a Western blot was performed to detect the expression of SphK1,S1P,MMP-13,and ADAMTS-5 proteins in CHON-001 cells.Results:MiR-613 could target the negative regulation of SphK1.Compared to the Ctrl group,the IL-1β group showed decreased EdU-positive cell rate,OD570 value,and miR-613 expression,while apoptosis rate,levels of IL-6,TNF-α,SphK1 mRNA and protein,S1P mRNA and protein,MMP-13,and ADAMTS-5 were increased(P<0.05).Compared to the IL-1β group and the NC-mimics group,the miR-613-mimics group exhibited increased EdU-positive cell rate,OD570 value,and miR-613 expression,whereas the apoptosis rate,levels of IL-6,TNF-α,SphK1 mRNA and protein,S1P mRNA and protein,MMP-13,and ADAMTS-5 were decreased(P<0.05).Compared to the miR-613-mimics group and the miR-613-mimics+OE-NC group,the miR-613-mimics+OE-SphK1 group had decreased EdU-positive cell rate and OD570 value,while apoptosis rate,levels of IL-6,TNF-α,SphK1 mRNA and protein,S1P mRNA and pro-tein,MMP-13,and ADAMTS-5 were increased(P<0.05).Conclusion:MiR-613 may inhibit IL-1β-in-duced chondrocyte injury by suppressing the SphK1/S1P pathway.关键词
软骨细胞损伤/微小RNA-613/鞘氨醇激酶1/鞘氨醇-1-磷酸/白细胞介素-1βKey words
Chondrocyte injury/MicroRNA-613/Sphingosine kinase 1/Sphingosine-1-phos-phate/Interleukin-1β引用本文复制引用
张佚舟,孙承军..miR-613调控SphK1/S1P通路对IL-1β诱导的软骨细胞损伤的影响[J].河北医学,2026,32(4):542-549,8.基金项目
湖北省武汉市医学科研项目,(编号:WX21D83) （编号:WX21D83）
