河北医学2026，Vol.32Issue(4)：549-558,10.DOI:10.3969/j.issn.1006-6233.2026.04.04
LncRNA MIR4435-2HG调节miR-889-3p/FOXK1轴对卵巢癌细胞增殖迁移和侵袭的影响
The Effect of LncRNA MIR4435-2HG in Regulating the miR-889-3p/FOXK1 Axis on the Proliferation Migration and Invasion of Ovarian Cancer Cells
摘要
Abstract
Objective:To investigate whether MIR4435-2HG can regulate the miR-889-3p/FOXK1 ax-is and affect the proliferation,migration,and invasion of ovarian cancer(OC)cells.Methods:RT-PCR was used to detect the expression levels of MIR4435-2HG,miR-889-3p,and FOXK1 mRNA in OC tissues,adja-cent cancer tissues,ovarian epithelial cells,and OC cells.The targeting relationship between miR-889-3p,MIR4435-2HG,and FOXK1 was validated.A2780 cells were assigned into NC group,sh-NC group,sh-MIR4435-2HG group,miR-NC group,miR-889-3p mimics group,sh-MIR4435-2HG+anti-NC group,sh-MIR4435-2HG+anti-miR-889-3p group,miR-889-3p mimics+pc-NC group,and miR-889-3p mimics+pc-FOXK1 group.The expression levels of MIR4435-2HG,miR-889-3p,and FOXK1 mRNA,cell prolifera-tion,migration,invasion,the protein expression levels of MMP-9,PD-L1,CyclinD1,FOXK1,E-cadherin,N-cadherin,and vimentin were measured.In vivo experiments were used to detect the effect of silencing MIR4435-2HG expression on the growth of ovarian tumors.Results:Compared with adjacent cancer tissues,the MIR4435-2HG and FOXK1 mRNA FOXK1 mRNA in OC tissues increased,while miR-889-3p decreased(P<0.05).Compared with IOSE-80 cells,the MIR4435-2HG and FOXK1 mRNA in OC cell line increased,while miR-889-3p decreased(P<0.05).Compared with the NC group and sh-NC group,the sh-MIR4435-2HG group showed decreased MIR4435-2HG and FOXK1 mRNA expression levels,cell viability,scratch heal-ing rate,number of cell invasions,MMP-9,N-cadherin,vimentin,PD-L1,FOXK1,and CyclinD1 protein ex-pression levels in A2780 cells,and increased miR-889-3p and E-cadherin protein expression levels(P<0.05).Compared with the miR-NC group,the miR-889-3p mimics group showed decreased FOXK1 mRNA ex-pression level,cell viability,scratch healing rate,number of cell invasions,MMP-9,N-cadherin,vimentin,PD-L1,FOXK1,and CyclinD1 protein expression levels in A2780 cells,and increased miR-889-3p and E-cad-herin protein expression levels(P<0.05).Downregulation of miR-889-3p expression was able to reduce the inhibitory effect of knocking down MIR4435-2HG expression on A2780 cells(P<0.05).Upregulation of FOXK1 was able to weaken the inhibitory effect of overexpression of miR-889-3p on A2780 cells(P<0.05).Compared with the sh-NC group,the tumor mass of nude mice in the sh-MIR4435-2HG group,MIR4435-2HG,FOXK1 mRNA and protein expression levels were decreased,the tumor volume was reduced,and the ex-pression level of miR-889-3p was increased(P<0.05).Conclusion:Knockdown of MIR4435-2HG expres-sion may inhibit A2780 cells by regulating the miR-889-3p/FOXK1 axis.关键词
卵巢癌/MIR4435-2HG/miR-889-3p/FOXK1轴/增殖/迁移/侵袭Key words
Ovarian cancer/MIR4435-2HG/miR-889-3p/FOXK1 axis/Proliferation/Mi-gration/Invasion引用本文复制引用
王爱芹,罗志中,左卫微,薛书霞..LncRNA MIR4435-2HG调节miR-889-3p/FOXK1轴对卵巢癌细胞增殖迁移和侵袭的影响[J].河北医学,2026,32(4):549-558,10.基金项目
河北省医学科学研究重点课题计划项目,(编号:20181214) （编号:20181214）
