河北医学2026，Vol.32Issue(4)：572-579,8.DOI:10.3969/j.issn.1006-6233.2026.04.07
miR-340-5p通过调控JAK1/STAT3通路对心肌梗死大鼠免疫功能及心功能的影响
Impacts of miR-340-5p on Immune Function and Cardiac Function in Myocardial Infarction Rats by Regulating JAK1/STAT3 Pathway
摘要
Abstract
Objective:To investigate the impacts of miR-340-5p on immune and cardiac functions in myocardial infarction(MI)rats by regulating JAK kinase 1(JAK1)/signal transducer and activator of tran-scription 3(STAT3)pathway.Methods:A myocardial infarction(MI)rat model was established by ligating the left anterior descending coronary artery,and assigned into sham operation group,MI group,MI+NC group,MI+miR-340-5p mimics group,and MI+miR-340-5p mimics+JAK1 inhibitor filgotinib(FILGO1)group.The cardiac function of MI rats was evaluated.TTC staining was used to determine the myocardial infarction ar-ea in MI rats.HE staining was performed to detect the degree of myocardial lesions.ELISA method was per-formed to measure inflammatory factors TNF-α,IL-1α,and IL-6.Flow cytometry was performed to determine the proportion of lymphocyte subsets.TUNEL method was used to detect cell apoptosis.qRT-PCR was used to detect the mRNA levels of miR-340-5p,JAK1,and STAT3 in myocardial tissue.Western blot was used to de-tect the expression of JAK1/STAT3 pathway-related proteins in myocardial tissue.In addition,dual luciferase reporter gene assay was used to validate the relationship between miR-340-5p and JAK1.Results:For the sham surgery group,the myocardial cells of rats in MI group showed hypertrophy,great increase in cell volume,and irregular morphology,the infarct size,CD8+,IL-6,IL-1α,TNF-α expression levels,myocardial cell apop-tosis rate,JAK1,STAT3 mRNA,p-JAK1/JAK1,p-STAT3/STAT3 levels raised,while LVEF,LVFS,CD4+,miR-340-5p decreased(P<0.05).For the MI group and MI+NC group,the myocardial cells of rats in MI+miR-340-5p mimics group and the MI+miR-340-5p mimics+FILGO1 group were arranged neatly and had clear textures,the infarct size,CD8+,IL-6,IL-1α,TNF-α levels,myocardial cell apoptosis rate,JAK1,STAT3 mRNA,p-JAK1/JAK1,p-STAT3/STAT3 levels decreased,while LVEF,LVFS,CD4+,miR-340-5p raised(P<0.05),and the MI+miR-340-5p mimics+FILGO1 group had larger changes in above indicators than the MI+miR-340-5p mimics group(P<0.05).Dual luciferase assay revealed a targeted relationship between miR-340-5p and JAK1(P<0.05).Conclusion:MiR-340-5p improves the immune and cardiac functions of MI rats by regulating JAK1/STAT3 pathway.关键词
心肌梗死/miR-340-5p/JAK激酶1/信号转导子和转录激活子3通路/免疫功能/心功能Key words
Myocardial infarction/miR-340-5p/JAK kinase 1/signal transducer and activator of transcription 3 Pathway/Immune function/Cardiac function引用本文复制引用
靳丹丹,李珊,刘沙沙,罗宏玉,李洁,史炜林,王伟..miR-340-5p通过调控JAK1/STAT3通路对心肌梗死大鼠免疫功能及心功能的影响[J].河北医学,2026,32(4):572-579,8.基金项目
河北省医学科学研究课题计划项目,(编号:20261384) （编号:20261384）
