河北医学2026，Vol.32Issue(4)：579-585,7.DOI:10.3969/j.issn.1006-6233.2026.04.08
骨髓间充质干细胞外泌体通过miR-133a/BMP-2轴调控大鼠前交叉韧带重建后腱骨愈合
Exosomes Derived from Bone Marrow-Derived Mesenchymal Stem Cells Regulate Tendon Bone Healing Following Anterior Cruciate Ligament Reconstruction in Rats via the miR-133a/BMP-2 Axis
摘要
Abstract
Objective:To investigate the effect of bone marrow mesenchymal stem cells(BMSCs)exo-somes on tendon-bone healing in rats after anterior cruciate ligament reconstruction,based on the miR-133a/BMP-2 axis.Methods:The SD rats were randomly divided into control group,exosome group,miR-NC exo-some group,miR-133a inhibitor exosome group,and miR-133a mimic exosome group,with 8 rats in each group.A rat model of anterior cruciate ligament reconstruction was constructed by performing anterior cruciate ligament reconstruction after the anterior cruciate ligament was severed.Micro-CT was used to detect bone vol-ume fraction(BV/TV),trabecular thickness,and cross-sectional area of the bone tunnel.The biomechanical test was performed to detect the maximum mechanical load of the reconstructed ligament in each group of rats.HE staining was used to detect the pathological changes of the tendon-bone interface.Saffron O-green staining was used to detect the formation of fibrocartilage in tendonosal interface tissues.Immunohistochemical staining was used to detect the expression and localization of BMP-2 protein in tendon interfacial tissue.Results:Com-pared with the control group,the BV/TV,trabecular bone thickness,maximum mechanical load of ligaments,formation of tendonosio-bone interface fibrocartilage,and BMP-2 increased in the exosomes group(P<0.05),and the cross-sectional area of the bone tunnel and the width of the tendon-bone interface decreased(P<0.05).Compared with the exosome group,there was no significant change in the above pathological indices in the miR-NC exosome group(P>0.05).Compared with the miR-NC exosomes group,the BV/TV,trabecu-lar bone thickness,maximum mechanical load of ligaments,formation of tendonosio-bone interface fibrocarti-lage,and BMP-2 increased in miR-133a inhibitor exosomes group(P<0.05),and the cross-sectional area of the bone tunnel and the width of the tendon-bone interface decreased(P<0.05);whereas the BV/TV,tra-becular bone thickness,maximum mechanical load of ligaments,formation of tendonosio-bone interface fibro-cartilage,and BMP-2 decreased in miR-133a mimic exosomes group(P<0.05),and the cross-sectional area of the bone tunnel and the width of the tendon-bone interface increased(P<0.05).Conclusion:Exosomes derived from BMSCs can encapsulate and deliver miR-133a to inhibit BMP-2 expression,thereby suppressing the patellar healing process in rats undergoing anterior cruciate ligament reconstruction.关键词
骨髓间充质干细胞/外泌体/前交叉韧带重建/腱骨愈合/miR-133a/BMP-2Key words
Bone marrow mesenchymal stem cells/Exosomes/Anterior cruciate ligament recon-struction/Tendon bone healing/miR-133a/BMP-2引用本文复制引用
李海清,尹帅,刘媛媛,张昕悦,李永犇,张海峰,宋效雷..骨髓间充质干细胞外泌体通过miR-133a/BMP-2轴调控大鼠前交叉韧带重建后腱骨愈合[J].河北医学,2026,32(4):579-585,7.基金项目
河北省医学科学研究课题计划资助,(编号:20220038) （编号:20220038）
