河北医学2026，Vol.32Issue(4)：586-593,8.DOI:10.3969/j.issn.1006-6233.2026.04.09
基于NF-κB/COX-2通路探讨桃红四物汤对大鼠下肢深静脉血栓形成的影响
A Study on the Effects of Taohong Siwu Decoction on Lower Extremity Deep Vein Thrombosis of Rats via the NF-κB/COX-2 Pathway
摘要
Abstract
Objective:To explore the effect of Taohong Siwu decoction(THSWD)on lower extremity deep vein thrombosis(LEDVT)in rats via the nuclear factor-kappa B(NF-κB)/cyclooxygenase-2(COX-2)pathway.Methods:The LEDVT rat model was constructed,and successfully modeled rats were randomly separated into LEDVT group,low-dose THSWD group(L-THSWD group),high-dose THSWD group(H-THSWD group),NF-κB inhibitor group(BAY11-7082 group),and high-dose THSWD+NF-κB/COX-2 ac-tivator phorbol 12-myristate 13-acetate group(H-THSWD+PMA group).An equal number of normal healthy SD rats were randomly selected to form the NC group(standard diet)and the sham surgery group(sham group).Changes in serum coagulation,hemorheology,and inflammatory cytokine-related indicators were meas-ured.The appearance of lower limb venous thrombosis in rats was observed,and wet weight was measured.The changes in inferior vena cava thrombosis and endothelial cell apoptosis were measured.The expression of tissue factor(TF)and NF-κB/COX-2 pathway-related proteins in the inferior vena cava thrombosis of rats in each group was detected.Results:Compared with the NC group and the sham group,the LEDVT group showed en-dothelial cell damage,increased inflammatory cell infiltration,luminal thrombosis,decreased activated partial thromboplastin time(APTT),thrombin time(TT),prothrombin time(PT),and increased fibrinogen(FIB),plasma viscosity,hematocrit,IL-6,IL-8,IL-1β,thrombus wet weight,endothelial cell apoptosis rate,TF mean optical density values,p-NF-κB p65/NF-κB p65,and COX-2 expression(P<0.05).Compared with the LEDVT group,the L-THSWD group,H-THSWD group,and BAY11-7082 group showed reduced endothelial cell damage,inflammatory cell infiltration,and luminal thrombus formation in rats,increased APTT,TT,and PT,and reduced FIB,plasma viscosity,hematocrit,IL-6,IL-8,IL-1β,thrombus wet weight,endothelial cell apoptosis rate,TF mean optical density values,p-NF-κB p65/NF-κB p65,and COX-2 expression(P<0.05).Compared with the H-THSWD group,the H-THSWD+PMA group showed increased endothelial cell damage,inflammatory cell infiltration,and luminal thrombus formation in rats,reduced APTT,TT,and PT,and increased FIB,plasma viscosity,hematocrit,IL-6,IL-8,IL-1β,thrombus wet weight,endothelial cell apopto-sis rate,TF mean optical density values,p-NF-κB p65/NF-κB p65,and COX-2 expression(P<0.05).Conclusion:TSD may increase LEDVT in rats by inhibiting the NF-κB/COX-2 pathway and suppressing the inflammatory response.关键词
下肢深静脉血栓形成/核转录因子κB/环氧合酶-2通路/桃红四物汤Key words
Lower extremity deep vein thrombosis/Nuclear factor-kappa B/cyclooxygenase-2 pathway/Taohong siwu decoction引用本文复制引用
李攀,田军军..基于NF-κB/COX-2通路探讨桃红四物汤对大鼠下肢深静脉血栓形成的影响[J].河北医学,2026,32(4):586-593,8.基金项目
湖北省中医药中医药科研联合基金项目,(编号:ZY2025L262) （编号:ZY2025L262）
