河北医学2026，Vol.32Issue(4)：614-621,8.DOI:10.3969/j.issn.1006-6233.2026.04.013
miR-181a-5p靶向DDIT4对UVB诱导的人皮肤成纤维细胞氧化损伤的影响
The Effect of miR-181a-5p Targeting DDIT4 on UVB-Induced Oxidative Damage in Human Skin Fibroblasts
摘要
Abstract
Objective:To investigate whether microRNA-181a-5p(miR-181a-5p)can regulate DNA Damage Inducible Transcript 4(DDIT4)to affect Ultraviolet B(UVB)induced oxidative damage in human skin fibroblasts(HSF).Methods:HSF were irradiated with UVB at 30mJ/cm2,and the expression level of miR-181a-5p was detected on 1d,3d,5d,and 7d,respectively.HSF was separated into normal control(NC)group,the UVB group,the miR-181a-5p low-expression negative control(anti-NC)group,the miR-181a-5p low-expression(anti-miR-181a-5p)group,the anti-miR-181a-5p+DDIT4 low-expression negative control(sh-NC)group,and the anti-miR-181a-5p+DDIT4 low-expression(sh-DDIT4)group.Real-time polymerase chain reaction(RT-PCR)was used to detect the miR-424-5p and DDIT4 mRNA in cells.Cell Counting Kit-8(CCK-8)was performed to detect cell viability.Flow cytometry was performed to detect cell apoptosis rate.The β-galactosidase staining method was used to observe cellular aging.The malon-dialdehyde(MDA),superoxide dismutase(SOD),and Catalase(CAT)in cells were detected.Protein im-munoblotting was used to detect the Matrix Metalloproteinase 9(MMP-9),Matrix Metalloproteinase 1(MMP-1),Cleaved cysteine-aspartic acid protease-3(cleaved caspase-3),and DDIT4 proteins.The targeting relationship between miR-181a-5p and DDIT4 was validated.Results:Compared with the control group,the UVB group had higher miR-181a-5p in HSF(P<0.05).Compared with the NC group,the UVB group had higher miR-181a-5p,apoptosis rate,proportion of senescent cells,MDA content,MMP-1,MMP-9,and cleaved caspase-3 proteins in HSF,and lower DDIT4 mRNA,cell viability,CAT and SOD activities,and DDIT4 protein(P<0.05).Compared with the UVB group and anti-NC group,the anti-miR-181a-5p group had lower miR-181a-5p,apoptosis rate,proportion of senescent cells,MDA content,MMP-1,MMP-9,and cleaved caspase-3 proteins in HSF,and higher DDIT4 mRNA,cell viability,CAT and SOD activities,and DDIT4 protein(P<0.05).Downregulation of DDIT4 expression could reduce the improvement effect of silencing miR-181a-5p expression on UVB-induced HSF oxidative damage(P<0.05).Conclusion:Silen-cing miR-181a-5p expression may alleviate UVB-induced oxidative damage in HSF by enhancing DDIT4 ex-pression.关键词
DNA损伤诱导转录物4/miR-181a-5p/中波紫外线/人皮肤成纤维细胞/氧化损伤Key words
DDIT4/miR-181a-5p/UVB/Human skin fibroblasts/Oxidative damage引用本文复制引用
张名,黄燕,吴希晞..miR-181a-5p靶向DDIT4对UVB诱导的人皮肤成纤维细胞氧化损伤的影响[J].河北医学,2026,32(4):614-621,8.基金项目
武汉市卫健委医学科学研究项目,(编号:WX23A07) （编号:WX23A07）
