郑州大学学报（医学版）2026，Vol.61Issue(2)：26-30,5.DOI:10.13705/j.issn.1671-6825.2025.03.151
胃癌组织和细胞中ZEB1和PD-L1表达的关系
Relation between the expression of ZEB1 and PD-L1 in gastric cancer tissues and cells
摘要
Abstract
Aim:To investigate the relation between zinc finger E-box binding homology box 1(ZEB1)and pro-grammed cell death-ligand 1(PD-L1)expression in gastric cancer tissues and cells.Methods:The expression levels of ZEB1 mRNA in 94 gastric cancer tissue microarrays(including 86 paired gastric cancer tissues and adjacent normal tissues)were detected using RNAscope technology,and PD-L1 protein expression was assessed by immunohistochemistry.Gastric cancer cell lines BGC-823 and AGS were divided into control group and interference group,and transfected with nonsense sequence or ZEB1-siRNA using Lipofectamine 3000.After 48 hours,the expression levels of ZEB1 and PD-L1 mRNA,pro-tein,and cell surface PD-L1 were detected by RT-qPCR,Western blot,and immunofluorescence,respectively.Results:ZEB1 was expressed in both gastric cancer cells and stroma,while PD-L1 was primarily localized in the gastric cancer stroma with minimal expression on the surface of gastric cancer cells.The positive expression rates of ZEB1 mRNA and PD-L1 protein in gastric cancer tissues were significantly higher than those in adjacent normal tissues(ZEB1:96.5％vs 86.0％,P=0.004;PD-L1:88.4％vs 29.1％,P<0.001),and showed a positive relation(rS=0.260,P=0.011)between them.The mRNA and protein expression levels of ZEB1 and PD-L1,as well as the cell surface PD-L1 expression of BGC-823 and AGS cells in the intervention group were significantly lower than those in the control group(P<0.05).Conclusion:ZEB1 and PD-L1 are highly expressed in gastric cancer tissues,and ZEB1 exerts a positive regulatory effect on PD-L1 in gastric cancer cells.关键词
锌指 E 盒结合同源盒1/程序性死亡配体1/胃癌Key words
zinc finger E-box binding homology box 1/programmed cell death-ligand 1/gastric cancer分类
医药卫生引用本文复制引用
叶春祥,杨磊,陈泓宇,李延森,王振军..胃癌组织和细胞中ZEB1和PD-L1表达的关系[J].郑州大学学报（医学版）,2026,61(2):26-30,5.基金项目
国家自然科学基金项目(81802353) （81802353）
