郑州大学学报（医学版）2026，Vol.61Issue(2)：36-40,5.DOI:10.13705/j.issn.1671-6825.2025.04.102
积雪草酸对乙型肝炎大鼠的干预效果及对肝组织 NF-κB/STAT3/ERK 通路的影响
Intervention effect of asiatic acid on rats with hepatitis B and its influence on NF-κB/STAT3/ERK signaling pathway in liver tissue
摘要
Abstract
Aim:To investigate the intervention effect of asiatic acid(AA)on the rats with hepatitis B and its influence on the NF-κB/STAT3/ERK signaling pathway in liver tissue.Methods:Sixty rats were randomly divided into normal control group,model group,low-,medium-,and high-dose AA groups(40,80,and 120 mg/kg),and positive control group(ente-cavir,0.5 mg/kg),with 10 rats in each group.Except for the normal control group,the other 5 groups were established as hepatitis B rat models by a single tail vein injection of 1.7×109 copy number rAAV-1.3HBV.Subsequently,the normal control group and the model group received intragastric administration of an equal volume of normal saline,while the other 4 groups received intragastric drug administration once daily for 10 days.Serum ALT and AST levels were detected using an automated biochemical analyzer;serum HA,LN,HBsAg,and HBeAg levels were measured by ELISA;HE staining was per-formed to observe hepatic histopathological changes;RT-qPCR was used to detect HBV DNA in the liver and serum sam-ples;and Western blot was employed to assess the phosphorylation levels of NF-κB p65,STAT3,and ERK proteins in liver tissue.Results:In the model group,hepatocytes showed disordered arrangement,swelling,necrosis,and significant inflam-matory cell infiltration.The three AA groups and the positive control group demonstrated reduced hepatocyte necrosis,gradu-ally uniform cell distribution,and decreased inflammatory cell infiltration.Serum ALT,AST,HA,and LN levels,as well as hepatic NF-κB p65,STAT3,and ERK protein phosphorylation levels in the model group were significantly elevated com-pared with the normal control group(P<0.05).Compared with the model group,the indicators mentioned above and HBV DNA load decreased in the medium-,high-dose AA groups and the positive control group(P<0.05),and no statistically significant difference was observed between the high-dose AA group and the positive control group(P>0.05).Conclu-sion:AA could inhibit HBV replication,and improve the liver tissue injury of rats with hepatitis B which may be associated with inhibiting the activation of NF-κB/STAT3/ERK signaling pathway.关键词
积雪草酸/乙型肝炎/NF-κB/STAT3/ERK 信号通路/大鼠Key words
asiatic acid/hepatitis B/NF-κB/STAT3/ERK signal pathway/rat分类
医药卫生引用本文复制引用
陈茂丽,韩成美,陈龙..积雪草酸对乙型肝炎大鼠的干预效果及对肝组织 NF-κB/STAT3/ERK 通路的影响[J].郑州大学学报（医学版）,2026,61(2):36-40,5.基金项目
山东中医药科技项目(2021M030) （2021M030）
