郑州大学学报（医学版）2026，Vol.61Issue(2)：82-86,5.DOI:10.13705/j.issn.1671-6825.2025.04.044
祛腐生肌合剂调节VEGF/PI3 K/Akt信号通路对大鼠慢性难愈性创面修复愈合的影响
Effects of Qufu Shengji mixture on repair and healing of chronic refracto-ry wound in rats by regulating VEGF/PI3 K/Akt signaling pathway
摘要
Abstract
Aim:To investigate the effects of Qufu Shengji mixture on the repair and healing of chronic refractory wound in rats by regulating the VEGF/PI3K/Akt signaling pathway.Methods:Sixty SD rats were randomly assigned to the sham operation group and the model group at a ratio of 1:5.After successful modeling,rats were randomly divided into the wound group,the traditional Chinese medicine group(1.35 g/kg Qufu Shengji mixture by gavage),the positive drug group(topical application of recombinant bovine basic fibroblast growth factor gel),the bevacizumab(Bmab)group(intraperitone-al injection of 5 mg/kg Bmab),and the traditional Chinese medicine+Bmab group(1.35 g/kg Qufu Shengji mixture by ga-vage,intraperitoneal injection of 5 mg/kg Bmab),with 10 rats in each group.After 8 days intervention,the wound healing rate of the rats was detected.The levels of TNF-α and IL-1β in the serum were determined by ELISA.The CD31 positive ar-ea percentage in the wound tissue was detected by immunohistochemistry.The mRNA expressions of transforming growth factor β1(TGF-β1)and basic fibroblast growth factor(bFGF)in the wound tissue were detected by qRT-PCR.The expres-sions of VEGF/PI3K/Akt pathway-related proteins in the wound tissue was detected by Western blot.Results:Compared with the sham operation group,the wound healing rate,the mRNA expression levels of TGF-β1 and bFGF,the CD31 positive area percentage,the protein expression level of VEGF,and the ratios of p-PI3K/PI3K and p-Akt/Akt in the wound tissue of the wound group decreased,while the levels of TNF-α and IL-1β in the serum increased(P<0.05).Compared with the wound group,the levels of TNF-α and IL-1β in the serum of the traditional Chinese medicine group and the positive drug group decreased,while the other indexes increased(P<0.05).Bmab weakened the repair and healing effects of the Qufu Shengji mixture on chronic refractory wounds in rats(P<0.05).Conclusion:Qufu Shengji mixture could promote the re-pair and healing of chronic refractory wound in rats by activating VEGF/PI3K/Akt signaling pathway.关键词
祛腐生肌合剂/VEGF/PI3K/Akt 信号通路/慢性难愈性创面/修复愈合/大鼠Key words
Qufu Shengji mixture/VEGF/PI3K/Akt signaling pathway/chronic refractory wound/repair and healing/rat分类
医药卫生引用本文复制引用
高飞,周泽高,李静,李景,唐淼..祛腐生肌合剂调节VEGF/PI3 K/Akt信号通路对大鼠慢性难愈性创面修复愈合的影响[J].郑州大学学报（医学版）,2026,61(2):82-86,5.基金项目
河北省中医药管理局科研计划项目(2025305) （2025305）
