河南农业大学学报2026，Vol.60Issue(2)：217-227,11.DOI:10.16445/j.cnki.1000-2340.20250407.005
红花种子脂肪酸代谢关键基因的挖掘与分析
The mining and analysis of key genes in fatty acid metabolism of Carthamus tinctorius L.seeds
摘要
Abstract
[Objective]To identify the key genes affecting fatty acid development in safflower seeds,so as to provide a theoretical basis and technical support for a deeper understanding of the molecular mechanisms of safflower fatty acid synthesis.[Method]Safflower seeds collected at 4,10,16,and 22 days after flowering(referred to as D4,D10,D16,and D22,respectively)were used as materials.The contents of 37 fatty acids in seeds at different developmental stages were measured.Transcriptome sequencing and bioinformatics analysis methods were employed to identify differentially expressed genes.Gene ontology(GO)and kyoto encyclopedia of genes and genomes(KEGG)enrichment analy-ses were conducted to explore the biological processes and biochemical metabolic pathways in which the differentially expressed genes in safflower seeds at different growth stages were involved.[Result]n the early stage of safflower seed development,saturated fatty acids accounted for 56.52%of the total fatty acids.After the 10th day after flowering,the content of linoleic acid increased from 5.03%to 75.41%.KEGG analysis indicated that the period from D4 to D10 was the critical stage for safflower fatty acid synthesis,with 37 differentially expressed genes enriched in the biosynthetic pathway of unsaturated fatty acids.The encoded products included stearoyl-CoA desaturase,omega-6 fatty acid desaturase,very-long-chain enoyl-CoA reductase,and acetyl-CoA acyltransferase.[Conclusion]A total of 29 genes involved in the fatty acid metabolic process of safflower seeds were identified.It was found that the expression of ACC,FATA,DGAT1 and FAD2 enzyme genes promoted the accumulation of fatty acids in safflower seeds.关键词
红花种子/转录组/游离脂肪酸/亚油酸Key words
Carthamus tinctorius L./transcriptome/free fatty acids/linoleic acids分类
农业科技引用本文复制引用
刘旸,苌建峰,张博,胡鹏,王溶溶,魏俊杰,洪利亚,李娟,李连珍..红花种子脂肪酸代谢关键基因的挖掘与分析[J].河南农业大学学报,2026,60(2):217-227,11.基金项目
国家自然科学基金青年基金项目(32300334) （32300334）
河南省高等学校青年骨干教师培养计划(2019GGJS045) （2019GGJS045）
2024年河南省中药材产业科技特派员服务团项目 （）
河南省中药材产业技术体系项目(HARS-22-11-G4) （HARS-22-11-G4）
