河南农业大学学报2026，Vol.60Issue(2)：251-262,12.DOI:10.16445/j.cnki.1000-2340.20250407.002
毛白杨RBOH基因家族鉴定与表达分析
Identification and expression analysis of RBOH gene family in Populus tomentosa
摘要
Abstract
[Objective]This study was conducted to identify members of the RBOH(respiratory burst oxidase homologue)family in Populus tomentosa and investigate their functions and roles in the growth and development of this species,thereby providing an informational foundation for stress resistance improvement in Populus tomentosa.[Method]The whole genome data of Populus tomentosa were sys-tematically analyzed,19 members of the RBOH family were identified,and their evolutionary relation-ships,gene structures,conserved motifs and domains,promoter cis-acting elements,and spatiotempo-ral expression levels were studied.[Results]The RBOH family members of Populus tomentosa are divided into 5 subfamilies,distributed on 11 chromosomes.Evolutionary analysis shows that RBOH members of Populus tomentosa have high homology with those of Arabidopsis and rice.The analysis of cis-acting elements shows that the promoter region of PtoRbohs in Populus tomentosa contains multiple elements that respond to light,hormones,and stress signals,indicating their possible involvement in various biological processes.Temporal and spatial expression analysis showed that PtoRboh4A was highly expressed in the roots of Populus tomentosa,while PtoRboh11D expression gradually increased with the degree of stem lignification.PtoRboh2D and PtoRboh3D were preferentially expressed in mature leaves.In the stem,PtoRboh4A was preferentially expressed in the phloem;PtoRboh1A,PtoR-boh1D and PtoRboh5A were preferentially expressed in the xylem.[Conclusion]The characteristics and expression patterns of the RBOH family members in Populus tomentosa were systematically ana-lyzed.The sequence and evolution of the RBOH family members in Populus tomentosa were relatively conservative,and they exhibited functional diversity.The high expression patterns of genes such as PtoRboh2D,PtoRboh3D,PtoRboh4A,and PtoRboh11D suggest that they may have important biologi-cal functions.关键词
毛白杨/RBOH/基因家族/基因表达/生物信息学Key words
Populus tomentosa/RBOH/gene family/gene expression/bioinformatics分类
农业科技引用本文复制引用
施润,吴雪珂,乐昱珅,郭朋,曹申全,尚富德..毛白杨RBOH基因家族鉴定与表达分析[J].河南农业大学学报,2026,60(2):251-262,12.基金项目
河南省自然科学基金项目(242300420483) （242300420483）
林木遗传育种全国重点实验室(中国林业科学研究院)开放课题基金项目(TGB-KF-2024-004) （中国林业科学研究院）
