河南农业大学学报2026，Vol.60Issue(2)：284-296,13.DOI:10.16445/j.cnki.1000-2340.20250813.001
紫云英优势根瘤菌的发掘与鉴定
Isolation and identification of dominant rhizobia associated with Astragalus sinicus
摘要
Abstract
[Objective]This study aimed to screen out the dominant strains from the Astragalus sinicus rhizobium resources in different region of China,so as to lay a solid foundation for the subsequent development and application of rhizobial inoculants.[Method]The symbiotic efficiency of 37 represen-tative Astragalus sinicus rhizobial strains previously isolated from Henan,Sichuan,Guangxi,Hubei and Hunan Provinces was determined and analyzed by the"double-pot"pot culture method in an artifi-cial climate chamber,with plant height,nodule number,leaf chlorophyll content and dry biomass as the key evaluation indicators.The strains with excellent symbiotic nitrogen fixation efficiency were selected for stress tolerance detection including acid-alkali resistance,salt tolerance and drought resis-tance.Furthermore,phylogenetic analysis based on the 16S rRNA gene,housekeeping gene atpD and nodulation gene nodC was conducted to clarify their taxonomic status.[Result]The optimal rhizobial strains for symbiosis with Astragalus sinicus in each province were identified as follows:WYCCWR 10750 and WYCCWR 10704 in Henan Province,which could tolerate a pH value range of 4-11,2%(mass fraction)NaCl and 7%(mass fraction)polyethylene glycol(PEG);WYCCWR 12312 and WYCCWR 12365 in Sichuan Province,which could tolerate a pH value range of 4-11,2%(mass frac-tion)NaCl and 5%(mass fraction)PEG;WYCCWR 12508 and WYCCWR 12418 in the Guangxi Zhuang Autonomous Region,which could tolerate a pH value range of 5-10,2%(mass fraction)NaCl and 7%(mass fraction)PEG;WYCCWR 12656 and WYCCWR 12542 in Hubei Province,which could tolerate a pH value range of 5-10,2%(mass fraction)NaCl and 7%(mass fraction)PEG;WYCCWR 12123 and WYCCWR 12091 in Hunan Province,which could tolerate a pH value range of 5-10,2%(mass fraction)NaCl and 7%(mass fraction)PEG.The above 10 strains were the domi-nant Astragalus sinicus rhizobia identified from different provinces in this study.Phylogenetic analysis results showed that all the 10 dominant strains belonged to the genus Mesorhizobium,and were identi-fied as three species including Mesorhizobium huakuii,Mesorhizobium qingshengii and Mesorhizobium jarvisii,with a single nodulation genotype.[Conclusion]A total of 10 dominant Astragalus sinicus rhizo-bial strains were excavated and identified from Henan,Sichuan,Guangxi,Hubei and Hunan Pro-vinces.All these strains could tolerate 2%(mass fraction)NaCl,had a wide acid-alkali tolerance range and a certain tolerance to polyethylene glycol,and contained conserved nodulation genes,which could provide excellent germplasm resources for the development of Astragalus sinicus rhizobial inoculants.关键词
紫云英/根瘤菌/高效固氮/抗逆性/系统发育分析Key words
Astragalus sinicus/Rhizobium/high efficient nitrogen fixation/stress resistance/phylo-genetic analysis分类
农业科技引用本文复制引用
李可,赵泽洋,杨柳,杨国平,张俊杰..紫云英优势根瘤菌的发掘与鉴定[J].河南农业大学学报,2026,60(2):284-296,13.基金项目
国家重点研发计划项目(2021YFD1700200) （2021YFD1700200）
