河南农业大学学报2026，Vol.60Issue(2)：307-315,9.DOI:10.16445/j.cnki.1000-2340.20250507.001
贝莱斯芽孢杆菌MD4抑制番茄灰霉病的生物防治潜力
Biocontrol potential of Bacillus velezensis MD4 in inhibiting tomato gray mold
摘要
Abstract
[Objective]To investigate the biocontrol potential of strain MD4 against tomato gray mold and its effect on fruit quality,and to provide theoretical support for the development and applica-tion of tomato gray mold microbial agents.[Method]The inhibitory effect of strain MD4 against Botrytis cinerea was determined by plate confrontation culture.The strain was identified through morphological,physiological and biochemical characteristics,and 16S rDNA sequence analysis.The control efficacy against tomato gray mold and the effects on the quality of postharvest tomato fruits were evaluated.[Result]1)Strain MD4 exhibited an inhibition rate of 70.40%against B.cinerea and was identified as Bacillus velezensis.2)MD4 treatment induced morphological abnor-malities in B.cinerea,including excessive hyphal branching,swelling,and cytoplasmic leakage.3)Inoculation with strain MD4 effectively reduced the incidence and disease index of tomato gray mold,with control efficacies of 65.31%and 55.21%for leaf and fruit infections,respectively.4)Strain MD4 could increase the contents of vitamin C,soluble solids,total phenols,and flavo-noids in tomato fruits,and delay the decrease in fruit weight loss rate caused by B.cinerea infec-tion.[Conclusion]Strain MD4 is a promising biocontrol strain with potential for application in tomato gray mold management.关键词
番茄灰霉病/贝莱斯芽孢杆菌/生物防治Key words
tomato gray mold/Bacillus velezensis/biological control分类
农业科技引用本文复制引用
郭翱翔,刘冬梅,裴冬丽,李志洋,祝春华,李萍,崔怡雪,张玉阳,韩霜,朱晓琴,张庆琛..贝莱斯芽孢杆菌MD4抑制番茄灰霉病的生物防治潜力[J].河南农业大学学报,2026,60(2):307-315,9.基金项目
河南省高等学校重点科研项目(25B180022) （25B180022）
河南省科技攻关项目(252102111098,262102111065) （252102111098,262102111065）
河南省良种攻关(20220100001) （20220100001）
河南省重大科技专项(221100110300) （221100110300）
